处理和/或盐水注射会改变大鼠纹状体和伏隔核中多巴胺代谢产物的基础水平以及吗啡诱发的变化。

Handling and/or saline injections alter basal and morphine-evoked changes in dopamine metabolites in the striatum and nucleus accumbens of rats.

作者信息

Johnson D W, Glick S D

机构信息

Department of Pharmacology and Toxicology, Albany Medical College, NY 12208.

出版信息

Pharmacol Biochem Behav. 1994 Mar;47(3):765-8. doi: 10.1016/0091-3057(94)90188-0.

DOI:10.1016/0091-3057(94)90188-0

PMID:8208798

Abstract

The effect of IP saline injections on basal dopamine (DA), homovanillic acid (HVA), and dihydroxyphenylacetic acid (DOPAC), and morphine-evoked levels of DA, DOPAC, and HVA in the striatum (STR) and nucleus accumbens (NAC) of female Sprague-Dawley rats was quantified, using in vivo microdialysis. In the STR, saline injections twice daily over 3 days, then once on day 4 (2x /day group), resulted in higher basal DOPAC and HVA levels compared to unhandled rats (naive group), or rats injected with saline once daily for 4 days (1x /day group). DOPAC and HVA were greater after IP injection of 30 mg/kg morphine in the saline 2x /day group compared to the saline 1x /day and naive groups. In the NAC, DOPAC was higher after injection of morphine in saline-pretreated rats, compared to naive rats. These results suggest that handling and/or IP saline injections affect basal and morphine-evoked changes in DA metabolites in the STR, and morphine-evoked DOPAC in the NAC.

摘要

采用体内微透析技术，对雌性斯普拉格-道利大鼠纹状体（STR）和伏隔核（NAC）中腹腔注射生理盐水对基础多巴胺（DA）、高香草酸（HVA）和二羟基苯乙酸（DOPAC）以及吗啡诱发的DA、DOPAC和HVA水平的影响进行了定量分析。在STR中，连续3天每天注射两次生理盐水，然后在第4天注射一次（每天2次组），与未处理的大鼠（未处理组）或连续4天每天注射一次生理盐水的大鼠（每天1次组）相比，基础DOPAC和HVA水平更高。与每天1次生理盐水组和未处理组相比，在每天2次生理盐水组腹腔注射30mg/kg吗啡后，DOPAC和HVA更高。在NAC中，与未处理大鼠相比，生理盐水预处理大鼠注射吗啡后DOPAC更高。这些结果表明，处理和/或腹腔注射生理盐水会影响STR中基础和吗啡诱发的DA代谢物变化，以及NAC中吗啡诱发的DOPAC变化。