Barrón B, Hernández A, Sandoval H
Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México, D.F., Mexico.
Rev Latinoam Microbiol. 1993 Apr-Jun;35(2):165-9.
Central neurons in culture represent a limitless substratum for research in neurobiology and experimental neurology. Primary cultures of NIH mouse neurons have shown that about 83% of total cells in the cultures are neuron clumps, detected by their reaction with the neuron specific-enolase (NSE) marker. Herpes Simplex Virus type 1 (HSV-1) can grow efficiently in these cultures, as it does in nonneuronal cultures usually used for antiviral drugs testing. For that reason, the primary neuronal cultures were used for testing antiviral activity against HSV-1, after an overnight treatment with different concentrations of dsRNA from phi 6 bacteriophage. The dsRNA started to be toxic for the cells at concentrations of 4 micrograms/ml, but it was found that 1 microgram/ml of this dsRNA protected all the neuronal cultures from HSV-1 infection. The dsRNA value for effective dose (ED50) was 0.27 microgram/ml.
培养的中枢神经元为神经生物学和实验神经学研究提供了无限的基础。国立卫生研究院（NIH）小鼠神经元的原代培养显示，培养物中约83%的细胞为神经元团块，可通过它们与神经元特异性烯醇化酶（NSE）标记物的反应来检测。1型单纯疱疹病毒（HSV-1）能在这些培养物中高效生长，就像它在通常用于抗病毒药物测试的非神经元培养物中一样。因此，在用来自phi 6噬菌体的不同浓度双链RNA过夜处理后，原代神经元培养物被用于测试抗HSV-1的抗病毒活性。双链RNA在浓度为4微克/毫升时开始对细胞产生毒性，但发现1微克/毫升的这种双链RNA可保护所有神经元培养物免受HSV-1感染。有效剂量（ED50）的双链RNA值为0.27微克/毫升。
