Glad H, Svendsen P, Ainsworth M A, Olsen O, Rehfeld J F, Schaffalitzky de Muckadell O B
Dept. of Medical Gastroenterology, Odense University Hospital, Denmark.
Scand J Gastroenterol. 1994 Mar;29(3):195-202. doi: 10.3109/00365529409090463.
The effect of gastrin-releasing peptide (GRP) (250, 500, 1000 pmol/kg.h) on the pancreaticobiliary bicarbonate secretion, the pancreatic protein secretion, and the plasma concentrations of secretin and cholecystokinin (CCK) was studied in the anaesthetized pig. Infusion of GRP (1000 pmol/kg.h) increased the portal plasma concentrations of secretin from 0.9 to 13.6 pmol/l and CCK from 1.2 to 38.4 pmol/l, the pancreatic bicarbonate secretion from 0.01 to 5.6 mmol/h, the hepatic bicarbonate secretion from 0.5 to 4.1 mmol/h, and the pancreatic protein secretion from 3 to 680 mg/h. Blocking of CCK-A receptors by MK-329 did not significantly change the effect of GRP, whereas prevention of secretin release by removal of the small intestine caused a 13-fold reduction in the GRP-induced pancreatic bicarbonate secretion and completely abolished the effect on hepatic bicarbonate secretion but did not change the effect on pancreatic protein secretion. We conclude that the effect of GRP on pancreaticobiliary bicarbonate secretion is not mediated through the release of CCK but more likely through the release of secretin and that the effect on pancreatic protein secretion is possibly a direct effect of GRP.
在麻醉猪中研究了胃泌素释放肽（GRP）（250、500、1000 pmol/kg·h）对胰胆管碳酸氢盐分泌、胰腺蛋白质分泌以及促胰液素和胆囊收缩素（CCK）血浆浓度的影响。输注GRP（1000 pmol/kg·h）可使门静脉血浆促胰液素浓度从0.9 pmol/l升高至13.6 pmol/l，CCK浓度从1.2 pmol/l升高至38.4 pmol/l，胰腺碳酸氢盐分泌从0.01 mmol/h增加至5.6 mmol/h，肝脏碳酸氢盐分泌从0.5 mmol/h增加至4.1 mmol/h，胰腺蛋白质分泌从3 mg/h增加至680 mg/h。MK-329阻断CCK-A受体并没有显著改变GRP的作用，而通过切除小肠来阻止促胰液素释放则使GRP诱导的胰腺碳酸氢盐分泌减少了13倍，并完全消除了对肝脏碳酸氢盐分泌的影响，但没有改变对胰腺蛋白质分泌的影响。我们得出结论，GRP对胰胆管碳酸氢盐分泌的作用不是通过CCK的释放介导的，而更可能是通过促胰液素的释放介导的，并且对胰腺蛋白质分泌的作用可能是GRP的直接作用。
