小鼠行为的正向和反向遗传学方法。
Forward and reverse genetic approaches to behavior in the mouse.
作者信息
Takahashi J S, Pinto L H, Vitaterna M H
机构信息
Department of Neurobiology and Physiology, Northwestern University, Evanston, IL 60208.
出版信息
Science. 1994 Jun 17;264(5166):1724-33. doi: 10.1126/science.8209253.
Abstract
Modern molecular genetic and genomic approaches are revolutionizing the study of behavior in the mouse. "Reverse genetics" (from gene to phenotype) with targeted gene transfer provides a powerful tool to dissect behavior and has been used successfully to study the effects of null mutations in genes implicated in the regulation of long-term potentiation and spatial learning in mice. In addition, "forward genetics" (from phenotype to gene) with high-efficiency mutagenesis in the mouse can uncover unknown genes and has been used to isolate a behavioral mutant of the circadian system. With the recent availability of high-density genetic maps and physical mapping resources, positional cloning of virtually any mutation is now feasible in the mouse. Together, these approaches permit a molecular analysis of both known and previously unknown genes regulating behavior.
摘要
现代分子遗传学和基因组学方法正在彻底改变小鼠行为学研究。通过靶向基因转移进行的“反向遗传学”（从基因到表型）提供了一个剖析行为的强大工具，并已成功用于研究与小鼠长期增强作用和空间学习调节相关基因的无效突变的影响。此外，在小鼠中进行高效诱变的“正向遗传学”（从表型到基因）可以发现未知基因，并已用于分离昼夜节律系统的行为突变体。随着最近高密度遗传图谱和物理图谱资源的可得性，在小鼠中对几乎任何突变进行定位克隆现在都是可行的。这些方法共同允许对调节行为的已知和以前未知的基因进行分子分析。
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本文引用的文献
1
Toward a physical map of the genome of the nematode Caenorhabditis elegans.构建秀丽隐杆线虫基因组物理图谱。
Proc Natl Acad Sci U S A. 1986 Oct;83(20):7821-5. doi: 10.1073/pnas.83.20.7821.
2
BEHAVIORAL MUTANTS OF Drosophila ISOLATED BY COUNTERCURRENT DISTRIBUTION.通过逆流分配法分离得到的果蝇行为突变体。
Proc Natl Acad Sci U S A. 1967 Sep;58(3):1112-9. doi: 10.1073/pnas.58.3.1112.
3
An augmenting effect of dose fractionation on radiation-induced mutation rate in mice.剂量分割对小鼠辐射诱导突变率的增强作用。
Proc Natl Acad Sci U S A. 1962 Oct 15;48(10):1724-7. doi: 10.1073/pnas.48.10.1724.
4
Circadian-clock regulation of gene expression.基因表达的昼夜节律调控。
Curr Opin Genet Dev. 1993 Apr;3(2):301-9. doi: 10.1016/0959-437x(93)90038-q.
5
TBASE: a computerized database for transgenic animals and targeted mutations.TBASE：一个用于转基因动物和定向突变的计算机化数据库。
Nature. 1993 May 27;363(6427):375-6. doi: 10.1038/363375a0.
6
Natural resistance to infection with intracellular parasites: isolation of a candidate for Bcg.对细胞内寄生虫感染的天然抵抗力：卡介苗候选物的分离
Cell. 1993 May 7;73(3):469-85. doi: 10.1016/0092-8674(93)90135-d.
7
Large deletions and other gross forms of chromosome imbalance compatible with viability and fertility in the mouse.与小鼠存活和生育能力相容的大片段缺失及其他染色体不平衡的总体形式。
Nat Genet. 1993 Jan;3(1):56-61. doi: 10.1038/ng0193-56.
8
Genetic analysis of circadian clocks.生物钟的遗传分析。
Annu Rev Physiol. 1993;55:683-728. doi: 10.1146/annurev.ph.55.030193.003343.
9
The mechanism of expression of long-term enhancement of hippocampal synapses: current issues and theoretical implications.海马体突触长期增强的表达机制：当前问题与理论意义
Annu Rev Physiol. 1993;55:375-96. doi: 10.1146/annurev.ph.55.030193.002111.
10
A yeast artificial chromosome covering the tyrosinase gene confers copy number-dependent expression in transgenic mice.一个覆盖酪氨酸酶基因的酵母人工染色体在转基因小鼠中赋予了拷贝数依赖性表达。
Nature. 1993 Mar 18;362(6417):258-61. doi: 10.1038/362258a0.
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