Handt O, Richards M, Trommsdorff M, Kilger C, Simanainen J, Georgiev O, Bauer K, Stone A, Hedges R, Schaffner W
Institute of Zoology, University of Munich, Germany.
Science. 1994 Jun 17;264(5166):1775-8. doi: 10.1126/science.8209259.
An approximately 5000-year-old mummified human body was recently found in the Tyrolean Alps. The DNA from tissue samples of this Late Neolithic individual, the so-called "Ice Man," has been extracted and analyzed. The number of DNA molecules surviving in the tissue was on the order of 10 genome equivalents per gram of tissue, which meant the only multi-copy sequences could be analyzed. The degradation of the DNA made the enzymatic amplification of mitochondrial DNA fragments of more than 100 to 200 base pairs difficult. One DNA sequence of a hypervariable segment of the mitochondrial control region was determined independently in two different laboratories from internal samples of the body. This sequence showed that the mitochondrial type of the Ice Man fits into the genetic variation of contemporary Europeans and that it was most closely related to mitochondrial types determined from central and northern European populations.
最近在蒂罗尔阿尔卑斯山发现了一具约有5000年历史的人类木乃伊。已从这位新石器时代晚期个体（即所谓的“冰人”）的组织样本中提取并分析了DNA。组织中存活的DNA分子数量约为每克组织10个基因组当量，这意味着只能分析唯一的多拷贝序列。DNA的降解使得酶促扩增超过100至200个碱基对的线粒体DNA片段变得困难。线粒体控制区高变段的一个DNA序列在两个不同实验室中分别从尸体内部样本独立测定。该序列表明，冰人的线粒体类型符合当代欧洲人的遗传变异，并且与从中欧和北欧人群中确定的线粒体类型关系最为密切。
