犬猫肠道冠状病毒3'端的基因组组织与表达

Genomic organization and expression of the 3' end of the canine and feline enteric coronaviruses.

作者信息

Vennema H, Rossen J W, Wesseling J, Horzinek M C, Rottier P J

机构信息

Department of Virology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.

出版信息

Adv Exp Med Biol. 1993;342:11-6. doi: 10.1007/978-1-4615-2996-5_2.

DOI:10.1007/978-1-4615-2996-5_2

PMID:8209715

Abstract

The genomic organization at the 3' end of canine coronavirus (CCV) and feline enteric coronavirus (FECV) was determined by sequence analysis and compared to that of feline infectious peritonitis virus (FIPV) and transmissible gastroenteritis virus (TGEV) of swine. Comparison of the latter two has previously revealed an extra open reading frame (ORF) at the 3' end of the FIPV genome, lacking in TGEV, now designated ORF 6b. Both CCV and FECV possess 6b-related ORFs. The CCV ORF 6b is colinear with that of FIPV, but the predicted amino acid sequences are only 58% identical. The FECV ORF 6b contains a large deletion compared to that of FIPV, reducing the colinear part to 60%. The sequence homologies were highest between CCV and TGEV on the one hand and between FECV and FIPV on the other. The expression product of the CCV and the FECV ORF 6b can be detected in infected cells by immunoprecipitation.

摘要

通过序列分析确定了犬冠状病毒（CCV）和猫肠道冠状病毒（FECV）3'端的基因组结构，并与猫传染性腹膜炎病毒（FIPV）和猪传染性胃肠炎病毒（TGEV）进行了比较。此前对后两者的比较显示，FIPV基因组3'端存在一个额外的开放阅读框（ORF），TGEV中没有，现在命名为ORF 6b。CCV和FECV都拥有与6b相关的ORF。CCV的ORF 6b与FIPV的共线，但预测的氨基酸序列只有58%相同。与FIPV相比，FECV的ORF 6b有一个大的缺失，使共线部分减少到60%。一方面，CCV和TGEV之间的序列同源性最高，另一方面，FECV和FIPV之间的序列同源性最高。通过免疫沉淀可以在感染细胞中检测到CCV和FECV ORF 6b的表达产物。