Gamble D A, Lobbiani A, Gramegna M, Moore L E, Colucci G
Animal Medical Center, New York, New York, USA.
J Clin Microbiol. 1997 Mar;35(3):673-5. doi: 10.1128/jcm.35.3.673-675.1997.
A diagnostic test for feline infectious peritonitis virus (FIPV) infection based on a nested PCR (nPCR) assay was developed and tested with FIPV, feline enteric coronavirus (FECV), canine coronavirus (CCV), and transmissible gastroenteritis virus (TGEV) and clinical fluid samples from cats with effusive feline infectious peritonitis (FIP). The target sequence for the assay is in the S1 region of the peplomer protein E2 gene. A vaccine strain of FIPV and two wild-type FIPV strains tested positive, but FECV, TGEV, and CCV tested negative. Preliminary tests with 12 cats with clinical evidence of effusive FIP and 11 cats with an illness associated with effusions, but attributed to other causes, were performed. Eleven of the 12 cats with effusive FIP tested positive, while 1 was negative. Ten of the 11 cats ill from other causes tested negative, while 1 was positive. On the basis of clinical laboratory and histopathologic criteria, the preliminary sensitivity and specificity of the assay were 91.6 and 94%, respectively.
开发了一种基于巢式聚合酶链反应(nPCR)检测的猫传染性腹膜炎病毒(FIPV)感染诊断试验,并使用FIPV、猫肠道冠状病毒(FECV)、犬冠状病毒(CCV)、传染性胃肠炎病毒(TGEV)以及来自渗出性猫传染性腹膜炎(FIP)猫的临床液体样本进行了测试。该检测的靶序列位于纤突蛋白E2基因的S1区域。一株FIPV疫苗株和两株野生型FIPV株检测呈阳性,但FECV、TGEV和CCV检测呈阴性。对12只具有渗出性FIP临床证据的猫和11只患有与积液相关疾病但归因于其他原因的猫进行了初步测试。12只渗出性FIP猫中有11只检测呈阳性,1只呈阴性。11只因其他原因患病的猫中有10只检测呈阴性,1只呈阳性。根据临床实验室和组织病理学标准,该检测的初步敏感性和特异性分别为91.6%和94%。