几种猫冠状病毒刺突基因的克隆与序列分析

Cloning and sequence analysis of the spike gene from several feline coronaviruses.

作者信息

Reed A P, Klepfer S, Miller T, Jones E

机构信息

Department of Molecular Biology, SmithKline Beecham Animal Health, King of Prussia, Pennsylvania 19406-0939.

出版信息

Adv Exp Med Biol. 1993;342:17-21. doi: 10.1007/978-1-4615-2996-5_3.

DOI:10.1007/978-1-4615-2996-5_3

PMID:8209725

Abstract

The DNA sequence encoding the spike gene from the DF2 strain of Type II feline infectious peritonitis virus (FIPV), a temperature sensitive FIPV virus (TS-DF2) and an isolate of feline enteric coronavirus (FECV 1683) were determined. Comparison of the published WSU 1146 and DF2 FIPV S genes showed that the viruses shared a high degree of homology (99.6%). Likewise, the S gene of the virulent DF2 FIPV virus was closely conserved to that isolated from the vaccine virus strain, TS-DF2 FIPV. In contrast, the FECV S gene had numerous DNA and amino acid differences when compared to the virulent FIPV sequences. Sequence differences among the feline coronavirus isolates were localized to the amino-terminus region of the S gene.

摘要

测定了II型猫传染性腹膜炎病毒（FIPV）DF2株、温度敏感型FIPV病毒（TS-DF2）以及猫肠道冠状病毒分离株（FECV 1683）的刺突基因编码DNA序列。已发表的WSU 1146和DF2 FIPV S基因的比较显示，这些病毒具有高度同源性（99.6%）。同样，强毒株DF2 FIPV病毒的S基因与从疫苗病毒株TS-DF2 FIPV分离出的S基因密切保守。相比之下，与强毒株FIPV序列相比，FECV S基因存在大量DNA和氨基酸差异。猫冠状病毒分离株之间的序列差异定位于S基因的氨基末端区域。