Opstelten D J, Horzinek M C, Rottier P J
Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
Adv Exp Med Biol. 1993;342:189-95. doi: 10.1007/978-1-4615-2996-5_30.
Using different approaches we have demonstrated the formation of a complex between the S protein and the M protein in the process of mouse hepatitis virus (MHV) assembly. Preservation of the M/S heterocomplexes was critically dependent on the solubilization conditions. Pulse-chase labeling of MHV-infected cells followed by a co-immunoprecipitation assay revealed that newly synthesized S and M engage in complex formation with different kinetics, the S protein reacting much slower. Sedimentation experiments showed the M/S heteromultimer complexes to be very large. A model for the role of the complex formation in MHV assembly is presented.
我们采用不同方法证明了在小鼠肝炎病毒（MHV）组装过程中，S蛋白和M蛋白之间形成了复合物。M/S异源复合物的保存情况严重依赖于溶解条件。对感染MHV的细胞进行脉冲追踪标记，随后进行共免疫沉淀分析，结果显示新合成的S蛋白和M蛋白以不同的动力学参与复合物形成，S蛋白反应慢得多。沉降实验表明M/S异源多聚体复合物非常大。本文提出了复合物形成在MHV组装中作用的模型。
