Nguyen V P, Hogue B G
Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030, USA.
Adv Exp Med Biol. 1998;440:361-5. doi: 10.1007/978-1-4615-5331-1_47.
Protein:protein interactions, and their subcellular localization, play important roles in coronavirus assembly. In this study, we have identified similar envelope glycoprotein complexes that are present in mouse hepatitis coronavirus A59 (MHV-A59) and bovine coronavirus (BCV) infected cells. Complexes consisting of the spike (S) and membrane (M) proteins were identified in cells infected with MHV-A59 or BCV. Kinetic analyses demonstrated that S and M quickly associated after translation, and suggested that both initially interacted in a pre-Golgi site. In addition, the hemagglutinin esterase (HE) was identified as part of a complex with M and S in BCV infected cells. Taken together, our data indicate that similar glycoprotein complexes are present in cells infected with two different coronaviruses, and thus likely represent important prerequisite complexes involved in virus assembly.
蛋白质与蛋白质之间的相互作用及其亚细胞定位在冠状病毒组装过程中发挥着重要作用。在本研究中，我们鉴定出了存在于感染小鼠肝炎冠状病毒A59（MHV-A59）和牛冠状病毒（BCV）的细胞中的相似包膜糖蛋白复合物。在感染MHV-A59或BCV的细胞中鉴定出了由刺突（S）蛋白和膜（M）蛋白组成的复合物。动力学分析表明，S蛋白和M蛋白在翻译后迅速结合，并提示两者最初在高尔基体前体部位相互作用。此外，在感染BCV的细胞中，血凝素酯酶（HE）被鉴定为与M蛋白和S蛋白形成复合物的一部分。综上所述，我们的数据表明，在感染两种不同冠状病毒的细胞中存在相似的糖蛋白复合物，因此它们可能代表了参与病毒组装的重要前提复合物。
