Collins A R
Department of Microbiology, State Univ. of NY at Buffalo 14214.
Adv Exp Med Biol. 1993;342:285-91. doi: 10.1007/978-1-4615-2996-5_44.
The binding of human coronavirus OC43 to human rhabdomyosarcoma cells which are highly susceptible to infection was studied by a solid phase virus binding assay and a receptor blockade assay. It was observed that whole virions and S(spike) bound to a 90 kD glycoprotein of RD cells even after treatment of the substrate with neuraminidase or 0.1 M NaOH. A second receptor of 45 kD also bound virus and was identified as HLA class I antigen. Antibody to both receptors reduced the virus yield in a receptor blockade assay. Sera from four patients with multiple sclerosis contained receptor blocking activity which correlated with antibodies to HLA. No receptor blocking antibodies to the 90 kD RD cell protein were found in human sera.
通过固相病毒结合试验和受体阻断试验研究了人冠状病毒OC43与对感染高度敏感的人横纹肌肉瘤细胞的结合。观察到，即使在用神经氨酸酶或0.1 M NaOH处理底物后，完整病毒粒子和S（刺突）仍与RD细胞的一种90 kD糖蛋白结合。一种45 kD的第二受体也能结合病毒，并且被鉴定为HLA I类抗原。在受体阻断试验中，针对这两种受体的抗体均降低了病毒产量。四名多发性硬化症患者的血清含有与抗HLA抗体相关的受体阻断活性。在人血清中未发现针对90 kD RD细胞蛋白的受体阻断抗体。
