Patton D L, Wolner-Hanssen P, Zeng W, Lampe M, Wong K, Stamm W E, Holmes K K
Department of Obsterics and Gynecology, University of Washington, Seattle 98195.
Sex Transm Dis. 1993 Jul-Aug;20(4):214-9.
The role of spermatozoa in carrying microorganisms from the cervix to the fallopian tubes and promoting infection was examined in eight pig-tailed macaques.
The aim of this study was to determine whether spermatozoa play a role in the transport of Chlamydia trachomatis into the upper reproductive tract and in the pathogenesis of chlamydial salpingitis.
Five monkeys were inoculated with Chlamydia trachomatis in the cervix and mated, and three other monkeys were infected in the cervix but not mated.
Culture positive results were obtained for cervical samples from all animals. After cervical inoculation, no mated animals and one unmated animal had positive culture results for samples from the fallopian tubes. After mating, no spermatozoa examined in samples taken from the upper tract or cul de sac had elementary bodies (EBs) on their surfaces, and only one sample obtained from the cervix showed C. trachomatis attached to the spermatozoa, as determined by fluorescent antibody (FA) staining. Two of the mated monkeys became pregnant; one spontaneously aborted late in gestation, and the other delivered a normal female infant. The fallopian tubes of all eight monkeys were challenged directly with serovar D and examined for evidence of salpingitis. Repeat laparoscopy was performed to monitor the inflammatory response. Tubal specimens for isolation and polymerase chain reaction (PCR) were obtained. Hysterectomies were performed between 3 and 4 weeks after tubal inoculation. Histopathologic examination of the fallopian tubes revealed widespread inflammation consisting of focal collections of lymphocytes and plasma cells in the submucosa. Peritubal adhesions were observed in four of five mated monkeys and in two of three unmated monkeys.
This study failed to confirm that spermatozoa play a role in the pathogenesis of C. trachomatis infection.
在八只豚尾猕猴中研究了精子在将微生物从子宫颈运送至输卵管并促进感染方面的作用。
本研究的目的是确定精子在沙眼衣原体向上生殖道的转运以及衣原体性输卵管炎的发病机制中是否起作用。
五只猴子在子宫颈接种沙眼衣原体后进行交配，另外三只猴子在子宫颈感染但未交配。
所有动物的子宫颈样本培养结果均为阳性。子宫颈接种后，未交配的动物中有一只以及所有交配的动物中均未检测到输卵管样本培养结果呈阳性。交配后，从上生殖道或阴道后穹隆采集的样本中检测的精子表面均未发现原体（EB），并且通过荧光抗体（FA）染色确定，仅从子宫颈获得的一个样本显示沙眼衣原体附着于精子。两只交配的猴子怀孕；一只在妊娠后期自然流产，另一只产下一名正常女婴。对所有八只猴子的输卵管直接用D血清型进行攻击，并检查输卵管炎的证据。进行重复腹腔镜检查以监测炎症反应。获取用于分离和聚合酶链反应（PCR）的输卵管标本。在输卵管接种后3至4周进行子宫切除术。输卵管的组织病理学检查显示广泛炎症，表现为黏膜下层淋巴细胞和浆细胞的局灶性聚集。五只交配猴子中的四只以及三只未交配猴子中的两只观察到输卵管周围粘连。
本研究未能证实精子在沙眼衣原体感染的发病机制中起作用。
