Simpson J W, Dreizen S
Tex Rep Biol Med. 1975;33(4):525-32.
Glucose utilization by the marmoset salivary glands was studied radioisotopically, both in vitro and in vivo. Surgically excised, minced parotid and submandibular glands from 7 adult animals were incubated in vitro in a medium containing uniformly labeled 14C glucose (glucose-UL-14C), then subjected to respirometry measurements and to extraction and separation procedures for the radioautographic isolation and identification of amino acids, organic acids, and glucose. Glucose utilization in vivo by the marmoset salivary glands was determined in 3 animals by analysis for 14C-containing amino acids, organic acids and glucose in pilocarpine-stimulated whole saliva, following intraperitoneal injection of glucose-UL-14C. The whole saliva was fractionated, separated and processed for localization of radioactivity by the same methods that were used in the in vitro studies. The isolated and in situ salivary glands converted glucose-UL-14C into 14C-containing carbon dioxide, glycine, alanine, glutamic acid, aspartic acid, and lactate. In no instance did the amount of 14C-glucose secreted in saliva exceed 0.52 mg/100 ml..
采用放射性同位素技术，对狨猴唾液腺在体外和体内的葡萄糖利用情况进行了研究。从7只成年动物身上手术切除腮腺和颌下腺并切碎，在含有均匀标记的14C葡萄糖（葡萄糖-UL-14C）的培养基中进行体外培养，然后进行呼吸测定，并对氨基酸、有机酸和葡萄糖进行提取、分离及放射自显影鉴定。通过腹腔注射葡萄糖-UL-14C后，对3只动物毛果芸香碱刺激产生的全唾液中含14C的氨基酸、有机酸和葡萄糖进行分析，测定狨猴唾液腺在体内的葡萄糖利用情况。全唾液通过与体外研究相同的方法进行分级、分离和放射性定位处理。离体和原位唾液腺将葡萄糖-UL-14C转化为含14C的二氧化碳、甘氨酸、丙氨酸、谷氨酸、天冬氨酸和乳酸。唾液中分泌的14C葡萄糖量在任何情况下均未超过0.52毫克/100毫升。
