Buszello H, Gabius H J, Ackermann R, Heicappell R
Department of Urology, Heinrich-Heine-Universität, Düsseldorf, Germany.
Urol Res. 1993;21(4):293-9. doi: 10.1007/BF00307715.
Frozen sections of primary and metastatic human renal cell carcinoma (RCC) were analyzed for the expression of endogenous binding sites for carbohydrates. Fluorescent neoglycoproteins, carrying chemically linked carbohydrate residues on bovine serum albumin as a carrier protein, were applied to 44 primary tumor specimens. In the majority of specimens, accessible binding sites with specificity for maltose and N-acetylgalactosamine were detected. In specimens of normal kidney no specific binding of carbohydrate ligands was observed under these experimental conditions. Specimens of both the primary tumor and a metastasis were available in 10 cases. When the expression of specific binding sites of primary tumors and metastases was compared, the respective patterns were similar with no clear gain or loss of certain lectins in the metastases. We conclude that binding sites with specificity for maltose and N-acetylgalactosamine are present on human RCC and their corresponding metastases.
对原发性和转移性人类肾细胞癌（RCC）的冰冻切片进行分析，以检测碳水化合物内源性结合位点的表达。将携带化学连接在牛血清白蛋白载体蛋白上的碳水化合物残基的荧光新糖蛋白应用于44个原发性肿瘤标本。在大多数标本中，检测到了对麦芽糖和N-乙酰半乳糖胺具有特异性的可及结合位点。在这些实验条件下，正常肾脏标本未观察到碳水化合物配体的特异性结合。10例患者同时有原发性肿瘤和转移灶的标本。当比较原发性肿瘤和转移灶特异性结合位点的表达时，各自的模式相似，转移灶中某些凝集素没有明显的增加或减少。我们得出结论，人类RCC及其相应转移灶上存在对麦芽糖和N-乙酰半乳糖胺具有特异性的结合位点。
