Tishon A, Eddleston M, de la Torre J C, Oldstone M B
Department of Neuropharmacology, Scripps Research Institute, La Jolla, California 92037.
Virology. 1993 Nov;197(1):463-7. doi: 10.1006/viro.1993.1613.
Lymphocytes and/or monocytes/macrophages carry viral genetic information in most, if not all, persistent and latent viral infections, and serve as potential reservoirs for maintaining or reintroducing the infection. Similarly, neurons can be persistently infected by several DNA and RNA viruses whose continued presence can alter the physiologic function of these cells, leading to disorders in neurotransmitters and disease. Here, we document that adoptive transfer of virus-specific cytotoxic T lymphocytes clears virus and viral nucleic acid sequences, in vivo, from individually infected lymphocytes, macrophages, and neurons. By plaquing, infectious center, Northern blot, and in situ hybridization at the single cell level, virus was efficiently removed from these cells.
在大多数（即便不是全部）持续性和潜伏性病毒感染中，淋巴细胞和/或单核细胞/巨噬细胞携带病毒遗传信息，并作为维持或重新引发感染的潜在储存库。同样，神经元可被多种DNA和RNA病毒持续感染，这些病毒的持续存在会改变这些细胞的生理功能，导致神经递质紊乱和疾病。在此，我们证明，病毒特异性细胞毒性T淋巴细胞的过继转移可在体内从单个受感染的淋巴细胞、巨噬细胞和神经元中清除病毒及病毒核酸序列。通过在单细胞水平进行噬斑测定、感染中心分析、Northern印迹和原位杂交，病毒被有效地从这些细胞中清除。
