Kim Y N, Jeong Y S, Makino S
Department of Microbiology, University of Texas at Austin 78712-1095.
Virology. 1993 Nov;197(1):53-63. doi: 10.1006/viro.1993.1566.
Mouse hepatitis virus (MHV) defective interfering (DI) RNA was used to determine the cis-acting sequences required for MHV RNA replication. A 2.2-kb-long cDNA clone of the MHV DI RNA DIssE was used to test the effect of deletions throughout the DI RNA on replication in DI RNA-transfected, MHV-infected cells. Data from a series of deletion mutants demonstrated that about 470 nucleotides at the 5' terminus, 460 nucleotides at the 3' terminus, and about 135 nucleotides in an internal position approximately 0.9 kb from the 5' end of DI RNA were necessary for DI RNA replication. These data suggested that cis-acting sequences which were necessary for MHV RNA replication required not only terminal sequences but also an internal sequence present at about 3.2 kb from the 5' end of the genome.
小鼠肝炎病毒（MHV）缺陷干扰（DI）RNA被用于确定MHV RNA复制所需的顺式作用序列。使用MHV DI RNA DIssE的一个2.2 kb长的cDNA克隆来测试DI RNA各处缺失对转染DI RNA的、感染MHV的细胞中复制的影响。一系列缺失突变体的数据表明，DI RNA复制需要DI RNA 5'端约470个核苷酸、3'端约460个核苷酸以及距离DI RNA 5'端约0.9 kb的内部位置的约135个核苷酸。这些数据表明，MHV RNA复制所需的顺式作用序列不仅需要末端序列，还需要基因组5'端约3.2 kb处存在的内部序列。
