红细胞保存研究。7. 用改良磷酸盐-铵添加剂溶液进行的体内和体外研究。

Studies in red blood cell preservation. 7. In vivo and in vitro studies with a modified phosphate-ammonium additive solution.

作者信息

Greenwalt T J, Dumaswala U J, Dhingra N, Allen C M, Silberstein E B

机构信息

Hoxworth Blood Center, University of Cincinnati Medical Center, Ohio.

出版信息

Vox Sang. 1993;65(2):87-94. doi: 10.1111/j.1423-0410.1993.tb02122.x.

DOI:10.1111/j.1423-0410.1993.tb02122.x

PMID:8212677

Abstract

Studies were conducted to examine whether an experimental additive solution (EAS-2) containing, in mM: 20 NH4Cl, 30 Na2HPO4, 2 adenine, 110 dextrose, 55 mannitol, pH 7.15, would be useful to extend the storage shelf life of human RBCs. With 6 pairs of split units, ATP concentrations were better maintained for 12 weeks with EAS-2 than with Adsol (1.8 vs. 1.1 mumol/g Hb, respectively, p = 0.002). Autologous 24-hours 51Cr viability values for split units in the same donors were: on 6 paired units at 8 weeks, EAS-2 87.0 +/- 4.5%, Adsol 72.6 +/- 2.3%, p = 0.004; on 11 paired units at 9 weeks, EAS-2 79.5 +/- 7.1%, Adsol 68.2 +/- 10.1%, p = 0.0003. The data suggest that packed RBCs stored for 9 weeks with EAS-2 will be suitable for transfusion following the removal of supernatant with a single washing step.

摘要

开展了多项研究，以检验一种实验性添加剂溶液（EAS-2）是否有助于延长人红细胞的储存保质期，该溶液含有（以毫摩尔计）：20氯化铵、30磷酸氢二钠、2腺嘌呤、110葡萄糖、55甘露醇，pH值为7.15。使用6对分离单位进行研究，结果显示，与Adsol相比，使用EAS-2可使ATP浓度在12周内得到更好的维持（分别为1.8与1.1微摩尔/克血红蛋白，p = 0.002）。同一供体的分离单位的自体24小时51铬活力值如下：在8周时对6对单位进行检测，EAS-2为87.0 +/- 4.5%，Adsol为72.6 +/- 2.3%，p = 0.004；在9周时对11对单位进行检测，EAS-2为79.5 +/- 7.1%，Adsol为68.2 +/- 10.1%，p = 0.0003。数据表明，使用EAS-2储存9周的浓缩红细胞，在经过单步洗涤去除上清液后将适合用于输血。