O'Hagan D T, Jeffery H, Davis S S
Department of Pharmaceutical Sciences, University of Nottingham, University Park, UK.
Vaccine. 1993;11(9):965-9. doi: 10.1016/0264-410x(93)90387-d.
Ovalbumin (OVA) was entrapped in microparticles prepared from three different poly(lactide-co-glycolide) (PLG) polymers and the microparticles were administered subcutaneously to mice as a single dose. Two weeks after immunization, the serum IgG antibody response to OVA entrapped in microparticles was significantly greater than the response to soluble OVA. The response to OVA entrapped in microparticles peaked at week 10 and remained high for the full 1-year duration of the study. In a second study, the effect of particle size on the immunogenicity of PLG microparticles with entrapped OVA was assessed. Following booster immunizations in mice, microparticles of 1.5 microns were significantly more immunogenic than microparticles of 72.6 microns. Furthermore, although enhanced serum IgG responses were induced by immunization with OVA adsorbed to microparticles (1.0 microns), entrapment of the OVA in microparticles (1.5 microns) resulted in significantly better responses.
卵清蛋白（OVA）被包裹于由三种不同的聚丙交酯-乙交酯（PLG）聚合物制备的微粒中，并将这些微粒以单剂量皮下注射给小鼠。免疫两周后，对包裹于微粒中的OVA的血清IgG抗体反应显著大于对可溶性OVA的反应。对包裹于微粒中的OVA的反应在第10周达到峰值，并在为期1年的整个研究期间保持高水平。在第二项研究中，评估了粒径对包裹有OVA的PLG微粒免疫原性的影响。在小鼠进行加强免疫后，1.5微米的微粒比72.6微米的微粒免疫原性显著更强。此外，尽管用吸附于微粒（1.0微米）的OVA免疫可诱导血清IgG反应增强，但将OVA包裹于微粒（1.5微米）中可产生显著更好的反应。
