Watanabe B I, Premaratne S, Limm W, Mugiishi M M, McNamara J J
Department of Surgery, Queens Medical Center, John A. Burns School of Medicine, University of Hawaii, Honolulu 96813.
Am Heart J. 1993 Oct;126(4):840-6. doi: 10.1016/0002-8703(93)90697-8.
Oxygen free radical scavengers have been found to decrease infarct size in dogs subjected to myocardial ischemia-reperfusion injury. A baboon open-chest model was used to determine if superoxide dismutase (SOD), an oxygen free radical scavenger, together with catalase would be equally effective in subhuman primates (baboons). The left anterior descending coronary artery (LAD) was ligated for 2 hours. Before reperfusion, the animals received the following: Group 1 (low-dose SOD/catalase; n = 5) received 15,000 IU/kg of SOD and 55,000 IU/kg of catalase IV over 1 hour, 15 minutes before reperfusion. Group 2 (high-dose human SOD [h-SOD]/catalase; n = 5) received an intraatrial bolus of 400,000 IU of recombinant h-SOD and 27,500 IU/kg of catalase over 30 seconds, followed by 300,000 IU of h-SOD and 55,000 IU/kg of catalase over 1 hour, beginning 15 seconds before reperfusion. Group 3 (n = 8) were control animals. Baboons were put to death 22 hours after reperfusion. Their hearts were excised and sectioned after the perfusion bed distal to the site of ligation was delineated with microvascular dye. The infarct zone was determined histologically. Areas of the perfusion bed and infarct zone were measured by planimetry. Infarct size did not differ significantly between the three groups: control, 66 +/- 7%; low-dose SOD/catalase, 68 +/- 5%; and high-dose h-SOD/catalase, 74 +/- 4%. In this model, high- and low-dose SOD with catalase did not result in any significant reduction in infarct size.
已发现氧自由基清除剂可减小遭受心肌缺血再灌注损伤的犬类的梗死面积。使用狒狒开胸模型来确定超氧化物歧化酶（SOD）（一种氧自由基清除剂）与过氧化氢酶联合使用在非人灵长类动物（狒狒）中是否同样有效。左冠状动脉前降支（LAD）结扎2小时。在再灌注前，动物接受以下处理：第1组（低剂量SOD/过氧化氢酶；n = 5）在再灌注前15分钟，于1小时内静脉注射15,000 IU/kg的SOD和55,000 IU/kg的过氧化氢酶。第2组（高剂量人SOD[h-SOD]/过氧化氢酶；n = 5）在再灌注前15秒开始，在30秒内于心房内推注400,000 IU的重组h-SOD和27,500 IU/kg的过氧化氢酶，随后在1小时内静脉注射300,000 IU的h-SOD和55,000 IU/kg的过氧化氢酶。第3组（n = 8）为对照动物。在再灌注22小时后处死狒狒。在用微血管染料勾勒出结扎部位远端的灌注床后，切除心脏并切片。通过组织学确定梗死区域。用面积测量法测量灌注床和梗死区域的面积。三组之间梗死面积无显著差异：对照组为66±7%；低剂量SOD/过氧化氢酶组为68±5%；高剂量h-SOD/过氧化氢酶组为74±4%。在该模型中，高剂量和低剂量的SOD与过氧化氢酶联合使用并未使梗死面积显著减小。
