Matsuda J, Gohchi K, Tsukamoto M, Saitoh N, Asami K, Hashimoto M
Department of Medicine, Teikyo University School of Medicine, Tokyo, Japan.
Am J Hematol. 1993 Nov;44(3):187-91. doi: 10.1002/ajh.2830440309.
We studied whether or not an anti-beta 2-glycoprotein I antibody (aGPI) had lupus anticoagulant-like activity, employing the diluted Russel viper venom time (dRVVT) and kaolin clotting time (KCT) as indices. aGPI prolonged the dRVVT and KCT of beta 2-glycoprotein I (GPI)-depleted normal plasma in the presence of extrinsic GPI. This prolongation of the dRVVT and KCT occurred immediately after the addition of aGPI and GPI, and was subsequently enhanced further in a time-dependent manner. The GPI/aGPI combination was judged to have lupus anticoagulant-like activity by the dRVVT-platelet neutralization test, but this was not confirmed by a lupus anticoagulant-specific test, i.e., the activated partial prothrombin time (APTT) using hexagonal phospholipid. From these findings, it can be concluded that aGPI has lupus anticoagulant-like activity in the presence of GPI, but may be a partially or considerably different antiphospholipid antibody from lupus anticoagulant. Further investigations may be needed to clarify this point.
我们以稀释的罗素蝰蛇毒时间（dRVVT）和高岭土凝血时间（KCT）为指标，研究了抗β2-糖蛋白I抗体（aGPI）是否具有狼疮抗凝物样活性。在存在外源性GPI的情况下，aGPI延长了β2-糖蛋白I（GPI）缺乏的正常血浆的dRVVT和KCT。dRVVT和KCT的这种延长在加入aGPI和GPI后立即发生，随后以时间依赖的方式进一步增强。通过dRVVT-血小板中和试验判断GPI/aGPI组合具有狼疮抗凝物样活性，但狼疮抗凝物特异性试验，即使用六角形磷脂的活化部分凝血活酶时间（APTT）未证实这一点。从这些发现可以得出结论，aGPI在存在GPI的情况下具有狼疮抗凝物样活性，但可能是一种与狼疮抗凝物部分或有很大不同的抗磷脂抗体。可能需要进一步研究来阐明这一点。
