Molinari J L, Soto R, Tato P, Rodriguez D, Retana A, Sepulveda J, Palet A
Instituto de Fisiologia Celular, Departamento de Microbiologia e Inmunologia, Universidad Nacional Autonoma de Mexico, Mexico City.
Am J Trop Med Hyg. 1993 Oct;49(4):502-12. doi: 10.4269/ajtmh.1993.49.502.
An antigenic extract from Taenia solium metacestodes was evaluated for immunogenicity in pig populations from a large area of endemic porcine cysticercosis in the State of Guerrero, Mexico. A total of 3,295 pigs from 18 villages were immunized with a single dose of 250 micrograms of protein administered intramuscularly. Systematic immunization was also performed on pigs (1,076 immunizations) from two of the villages with the highest percentages of cysticercosis. A year after immunization, porcine cysticercosis decreased from 4.8% and 5.4% to 0%. Immunity against the T. solium metacestode was estimated in vitro by measurements of 3H-thymidine uptake and inhibition of leukocyte migration. Peripheral blood lymphocytes from immunized cysticercotic (pigs that had cysticercosis prior to immunization), cysticercotic immunized (pigs that acquired cysticercosis after immunization), and normal control pigs incorporated 3H-thymidine better than lymphocytes from cysticercotic pigs when stimulated with concanavalin A. A significant inhibition in the leukocyte migration inhibition test was also found in leukocytes from immunized cysticercotic pigs (P < 0.01). Histopathologic studies revealed granuloma formation surrounding the metacestodes of the immunized cysticercotic and cysticercotic immunized pigs. These metacestodes exhibited several stages of destruction. Large numbers of eosinophils were frequently observed in a close association with the degeneration and destruction of parasites. Metacestodes in control cysticercotic pigs were intact and surrounded by a minor inflammatory reaction. Finally, the rate of in vitro evagination of scolices was high in metacestodes obtained from cysticercotic pigs and low or absent in those from immunized pigs (P < 0.01).
对来自墨西哥格雷罗州大面积猪囊尾蚴病流行区的猪群，评估了猪带绦虫囊尾蚴的一种抗原提取物的免疫原性。来自18个村庄的总共3295头猪接受了单剂量250微克蛋白质的肌肉注射免疫。还对囊尾蚴病发病率最高的两个村庄的猪（1076次免疫）进行了系统免疫。免疫一年后，猪囊尾蚴病发病率从4.8%和5.4%降至0%。通过测量3H-胸腺嘧啶核苷摄取和白细胞迁移抑制，在体外评估了对猪带绦虫囊尾蚴的免疫力。当用刀豆球蛋白A刺激时，来自免疫的囊尾蚴病猪（免疫前患有囊尾蚴病的猪）、囊尾蚴病免疫猪（免疫后感染囊尾蚴病的猪）和正常对照猪的外周血淋巴细胞比来自囊尾蚴病猪的淋巴细胞摄取3H-胸腺嘧啶核苷的能力更强。在免疫的囊尾蚴病猪的白细胞中，白细胞迁移抑制试验也发现有显著抑制（P<0.01）。组织病理学研究显示，免疫的囊尾蚴病猪和囊尾蚴病免疫猪的囊尾蚴周围形成了肉芽肿。这些囊尾蚴呈现出几个破坏阶段。经常观察到大量嗜酸性粒细胞与寄生虫的退化和破坏密切相关。对照囊尾蚴病猪的囊尾蚴完整，周围有轻微炎症反应。最后，从囊尾蚴病猪获得的囊尾蚴中头节的体外外翻率很高，而从免疫猪获得的囊尾蚴中头节的体外外翻率很低或没有（P<0.01）。
