Bertossi M, Roncali L, Nico B, Ribatti D, Mancini L, Virgintino D, Fabiani G, Guidazzoli A
Institute of Human Anatomy, Histology and Embryology, Policlinics, University of Bari, Italy.
Anat Embryol (Berl). 1993 Jul;188(1):21-9. doi: 10.1007/BF00191448.
The role played by perivascular astrocytes in neural vessel maturation was investigated in microvessels of the chick embryo optic tectum. Three-dimensional reconstructions and quantitative analyses were made, and permeability was studied. On embryonic days 14-16, 12.5% of the microvessel wall is surrounded by astrocyte endfeet which, in most cases (82%), are located under endothelium junctions; the latter, at this stage, partly prevent the extravascular escape of the marker horseradish peroxidase. On days 18-21, the astrocyte processes form a nearly complete perivascular sheath enveloping 96% of the microvessel perimeter; the junctions of the endothelial cells are much wider and impermeable owing to extensive fusion of the endothelial plasma membranes. This investigation suggests a close relationship between the perivascular arrangement of glia and differentiation of the endothelium tight junctions and indicates that the morphofunctional maturation of the latter takes place progressively during the prenatal organogenesis of the chick central nervous system.
在鸡胚视顶盖的微血管中研究了血管周围星形胶质细胞在神经血管成熟过程中所起的作用。进行了三维重建和定量分析，并研究了通透性。在胚胎第14 - 16天，12.5%的微血管壁被星形胶质细胞终足包围，在大多数情况下（82%），这些终足位于内皮连接下方；在此阶段，内皮连接部分阻止了标记物辣根过氧化物酶的血管外逸。在第18 - 21天，星形胶质细胞突起形成几乎完整的血管周围鞘，包绕96%的微血管周长；由于内皮细胞质膜的广泛融合，内皮细胞的连接更宽且具有不透性。这项研究表明神经胶质细胞的血管周围排列与内皮紧密连接的分化之间存在密切关系，并表明后者的形态功能成熟在鸡中枢神经系统产前器官发生过程中逐步发生。
