Cox R A, Godwin E, Hastings J R
Biochem J. 1976 Jun 1;155(3):465-75. doi: 10.1042/bj1550465a.
RNA was isolated from subribosomal particles of the malaria parasite Plasmodium knowlesi. The nucleotide composition (mole fraction) of the principal species was obtained (S-rRNA, 0.295A, 0.36U, 0.25G, 0.105C: L-rRNA, 0.326A, 0.31U, 0.228G, 0.144C). Ribosomal RNA was also isolated from Drosophila melanogaster. Optical properties of these A + U-rich species were measured. In all four cases analysis of the hypochromic effect revealed that adenine and uracil residues tended to form clusters along the polynucleotide chain. A substantial fraction of residues was located in bihelical regions of approx. 50% G-C base pairs or in regions of approx. 30-35% G-C base pairs. The possible evolutionary significance of these results was considered on the basis of comparison with properties of rRNA from bacteria (Escherichia coli) and a mammal (rabbit reticulocyte).
从诺氏疟原虫的亚核糖体颗粒中分离出RNA。获得了主要种类的核苷酸组成(摩尔分数)(小核糖体RNA,0.295A、0.36U、0.25G、0.105C;大核糖体RNA,0.326A、0.31U、0.228G、0.144C)。还从黑腹果蝇中分离出核糖体RNA。测量了这些富含A+U种类的光学性质。在所有四种情况下,对减色效应的分析表明,腺嘌呤和尿嘧啶残基倾向于沿着多核苷酸链形成簇。相当一部分残基位于约50%G-C碱基对的双螺旋区域或约30-35%G-C碱基对的区域。基于与来自细菌(大肠杆菌)和哺乳动物(兔网织红细胞)的rRNA特性的比较,考虑了这些结果可能的进化意义。