黑腹果蝇的核酸。
The nucleic acids of Drosophila melanogaster.
作者信息
Hastings J R, Kirby K S
出版信息
Biochem J. 1966 Aug;100(2):532-9. doi: 10.1042/bj1000532.
Abstract
- Nucleic acids of whole Drosophila adults were prepared in good yield and substantially free from impurities by new modifications of the phenol method. 2. The average molar base compositions of the DNA (41% of guanine+cytosine) and transfer RNA (60% of guanine+cytosine) resemble those of mammalian nucleic acids; the ribosomal RNA has a DNA-like molar base composition (43% of guanine+cytosine), and it is considered that this is reflected in the lower stability of its secondary structure compared with mammalian ribosomal RNA. 3. The two main ribosomal forms were separated and average base compositions and sedimentation values determined.
摘要
- 通过酚法的新改进方法，以良好的产率制备了完整果蝇成虫的核酸，且基本不含杂质。2. DNA（鸟嘌呤+胞嘧啶占41%）和转移RNA（鸟嘌呤+胞嘧啶占60%）的平均摩尔碱基组成与哺乳动物核酸的相似；核糖体RNA具有类似DNA的摩尔碱基组成（鸟嘌呤+胞嘧啶占43%），据认为，这反映出其二级结构与哺乳动物核糖体RNA相比稳定性较低。3. 分离出两种主要的核糖体形式，并测定了平均碱基组成和沉降值。
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2
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3
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