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镁离子对兔网织红细胞核糖体核糖核酸构象及较大核糖体亚基颗粒稳定性影响的研究。

A study of the influence of magnesium ions on the conformation of ribosomal ribonucleic acid and on the stability of the larger subribosomal particle of rabbit reticulocytes.

作者信息

Cox R A, Hirst W

出版信息

Biochem J. 1976 Dec 15;160(3):505-19. doi: 10.1042/bj1600505.

DOI:10.1042/bj1600505
PMID:797388
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1164267/
Abstract

Mg2+ was shown to affect the conformation of rRNA over the range of 0.03-1.2M-KCl. The species studies were Escherichia coli S-rRNA and L-rRNA (the RNA moieties of the smaller and larger subribosomal particles respectively) and rabbits S-rRNA and L-rRNA. 2. The addition of Mg2+ to rRNA in reconstitution buffer (0.35M-KCl0.01M-Tris/HCl, pH7.2) at 20 degrees C let to an increase in bihelical secondary structure through the formation of additional (mainly A-U) base-pairs (e.g. an additional approx. 58 A-U base-pairs per molecule of E. coli S-rRNA as judged by u.v. difference spectrophotometry...

摘要

已表明，在0.03 - 1.2M - KCl范围内，Mg2+会影响rRNA的构象。所研究的种类有大肠杆菌的S - rRNA和L - rRNA（分别为较小和较大核糖体亚基颗粒的RNA部分）以及兔的S - rRNA和L - rRNA。2. 在20℃下，于重构缓冲液（0.35M - KCl、0.01M - Tris/HCl，pH7.2）中向rRNA添加Mg2+，通过形成额外的（主要是A - U）碱基对，导致双螺旋二级结构增加（例如，通过紫外差示分光光度法判断，每分子大肠杆菌S - rRNA大约增加58个A - U碱基对……

相似文献

1
A study of the influence of magnesium ions on the conformation of ribosomal ribonucleic acid and on the stability of the larger subribosomal particle of rabbit reticulocytes.镁离子对兔网织红细胞核糖体核糖核酸构象及较大核糖体亚基颗粒稳定性影响的研究。
Biochem J. 1976 Dec 15;160(3):505-19. doi: 10.1042/bj1600505.
2
A study of the thermal stability of ribosomes and biologically active subribosomal particles.核糖体及具有生物活性的核糖体亚基颗粒的热稳定性研究。
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3
Re-activation of the peptidyltransferase centre of rabbit reticulocyte ribosomes after inactivation by exposure to low concentrations of magnesium ion.经低浓度镁离子处理而失活的兔网织红细胞核糖体肽基转移酶中心的重新激活。
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4
Secondary structure features of ribosomal RNA species within intact ribosomal subunits and efficiency of RNA-protein interactions in thermoacidophilic (Caldariella acidophila, Bacillus acidocaldarius) and mesophilic (Escherichia coli) bacteria.完整核糖体亚基内核糖体RNA种类的二级结构特征以及嗜热嗜酸菌（嗜酸热硫化叶菌、嗜酸芽孢杆菌）和嗜温菌（大肠杆菌）中RNA-蛋白质相互作用的效率。
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Reassembly of the peptidyltransferase centre of larger subparticles of rabbit reticulocyte ribosomes from a core-particle and split-protein fraction.从核心颗粒和裂解蛋白组分重新组装兔网织红细胞核糖体较大亚颗粒的肽基转移酶中心。
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The role of magnesium and potassium ions in the molecular mechanism of ribosome assembly: hydrodynamic, conformational, and thermal stability studies of 16 S RNA from Escherichia coli ribosomes.镁离子和钾离子在核糖体组装分子机制中的作用：来自大肠杆菌核糖体的16S RNA的流体动力学、构象及热稳定性研究
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Correlation of the expansion segments in mammalian rRNA with the fine structure of the 80 S ribosome; a cryoelectron microscopic reconstruction of the rabbit reticulocyte ribosome at 21 A resolution.哺乳动物核糖体RNA中扩展片段与80S核糖体精细结构的相关性；兔网织红细胞核糖体在21埃分辨率下的冷冻电子显微镜重建。
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A spectrophotometric study of the secondary structure of ribonucleic acid based on a method for diminishing single-stranded base-'stacking' without affecting multi-helical structures.基于一种在不影响多螺旋结构的情况下减少单链碱基“堆积”的方法对核糖核酸二级结构进行的分光光度研究。
Biochem J. 1967 Jun;103(3):749-58. doi: 10.1042/bj1030749.
9
Studies of the RNA and protein moieties of the larger subribosomal particle of rabbit reticulocytes.兔网织红细胞较大亚核糖体颗粒的RNA和蛋白质部分的研究。
Acta Biol Med Ger. 1974;33(5-6):733-52.
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The circular dichroism of ribosomal ribonucleic acids.核糖体核糖核酸的圆二色性
Biochem J. 1976 May 1;155(2):279-91. doi: 10.1042/bj1550279.

引用本文的文献

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Omnipotent decoding potential resides in eukaryotic translation termination factor eRF1 of variant-code organisms and is modulated by the interactions of amino acid sequences within domain 1.全能解码潜力存在于变码生物的真核翻译终止因子eRF1中，并受结构域1内氨基酸序列相互作用的调节。
Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8494-9. doi: 10.1073/pnas.142690099.
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Ribosomal proteins of Thermomyces lanuginosus--characterisation by two-dimensional gel electrophoresis and differential disassembly.嗜热栖热放线菌的核糖体蛋白——通过二维凝胶电泳和差异拆卸进行表征
Mol Cell Biochem. 1995 Feb 9;143(1):21-34. doi: 10.1007/BF00925923.
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Termination of translation in eukaryotes is governed by two interacting polypeptide chain release factors, eRF1 and eRF3.真核生物中翻译的终止由两个相互作用的多肽链释放因子eRF1和eRF3控制。
EMBO J. 1995 Aug 15;14(16):4065-72. doi: 10.1002/j.1460-2075.1995.tb00078.x.
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Reconstruction of the smaller subparticle of rabbit ribosomes from core-particle and split-protein fractions.利用核心颗粒和裂解蛋白组分重建兔核糖体的较小亚基颗粒
Biochem J. 1981 Mar 15;194(3):931-9. doi: 10.1042/bj1940931.
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Resistance of the peptidyltransferase centre of rabbit ribosomes to attack by nucleases and proteinases.兔核糖体肽基转移酶中心对核酸酶和蛋白酶攻击的抗性。
Biochem J. 1980 Jul 15;190(1):199-214. doi: 10.1042/bj1900199.
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Protein synthesis by hybrid ribosomes reconstructed from rabbit reticulocyte ribosomal core-particles and amphibian or fungal split-proteins.由兔网织红细胞核糖体核心颗粒与两栖类或真菌类裂解蛋白重建的杂种核糖体进行的蛋白质合成。
Biochem J. 1980 Mar 15;186(3):861-72. doi: 10.1042/bj1860861.
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Distribution of sequences common to the 25--28S-ribonucleic acid genes of Xenopus laevis and Neurospora crassa.非洲爪蟾和粗糙脉孢菌25-28S核糖体核糖核酸基因共有序列的分布
Biochem J. 1980 Apr 1;187(1):75-90. doi: 10.1042/bj1870075.
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Re-activation of the peptidyltransferase centre of rabbit reticulocyte ribosomes after inactivation by exposure to low concentrations of magnesium ion.经低浓度镁离子处理而失活的兔网织红细胞核糖体肽基转移酶中心的重新激活。
Biochem J. 1976 Dec 15;160(3):521-31. doi: 10.1042/bj1600521.
9
A study of the organisation of the ribosomal ribonucleic acid gene cluster of Neurospora crassa by means of restriction endonuclease analysis and cloning in bacteriophage lambda.通过限制性内切酶分析和噬菌体λ克隆对粗糙脉孢菌核糖体核糖核酸基因簇组织的研究。
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本文引用的文献

1
Ribonucleic acid from Escherichia coli. III. The influence of ionic strength and temperature on hydrodynamic and optical properties.来自大肠杆菌的核糖核酸。III. 离子强度和温度对流体动力学及光学性质的影响。
Biochim Biophys Acta. 1962 Aug 20;61:197-208.
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The effect of temperature on the magnesium binding and ultracentrifugal properties of rat liver ribosomes.
Biochemistry. 1967 Sep;6(9):2950-8. doi: 10.1021/bi00861a040.
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The dissociation of reticulocyte polysomes into subunits and the location of messenger RNA.网织红细胞多核糖体解离为亚基及信使核糖核酸的定位
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The function of high-molecular-weight ribonucleic acid from rabbit reticulocytes in haemoglobin biosynthesis.来自兔网织红细胞的高分子量核糖核酸在血红蛋白生物合成中的作用。
Biochem J. 1964 Sep;92(3):648-61. doi: 10.1042/bj0920648.
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Molecular weights of ribosomal RNA in relation to evolution.核糖体RNA分子量与进化的关系
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Conformation of nucleic acids and the analysis of the hypochromic effect.核酸的构象与减色效应分析
Biochem J. 1970 Dec;120(3):539-47. doi: 10.1042/bj1200539.
7
Dissociation of ribosomes from oocytes of Xenopus laevis into active subparticles.非洲爪蟾卵母细胞中的核糖体解离为活性亚颗粒。
Biochem J. 1971 Oct;124(5):897-903. doi: 10.1042/bj1240897.
8
Magnesium-induced conformational change in transfer ribonucleic acid as measured by circular dichroism.通过圆二色性测量镁诱导的转移核糖核酸构象变化。
Biochemistry. 1971 Jun 8;10(12):2216-22. doi: 10.1021/bi00788a005.
9
Assembly mapping of 30S ribosomal proteins from E. coli.来自大肠杆菌的30S核糖体蛋白的装配图谱
Nature. 1970 Jun 27;226(5252):1214. doi: 10.1038/2261214a0.
10
A spectrophotometric study of the secondary structure of ribonucleic acid isolated from the smaller and larger ribosomal subparticles of rabbit reticulocytes.对从兔网织红细胞的较小和较大核糖体亚颗粒中分离出的核糖核酸二级结构的分光光度研究。
Biochem J. 1970 Mar;117(1):101-18. doi: 10.1042/bj1170101.