镁离子对兔网织红细胞核糖体核糖核酸构象及较大核糖体亚基颗粒稳定性影响的研究。
A study of the influence of magnesium ions on the conformation of ribosomal ribonucleic acid and on the stability of the larger subribosomal particle of rabbit reticulocytes.
作者信息
Cox R A, Hirst W
出版信息
Biochem J. 1976 Dec 15;160(3):505-19. doi: 10.1042/bj1600505.
Abstract
Mg2+ was shown to affect the conformation of rRNA over the range of 0.03-1.2M-KCl. The species studies were Escherichia coli S-rRNA and L-rRNA (the RNA moieties of the smaller and larger subribosomal particles respectively) and rabbits S-rRNA and L-rRNA. 2. The addition of Mg2+ to rRNA in reconstitution buffer (0.35M-KCl0.01M-Tris/HCl, pH7.2) at 20 degrees C let to an increase in bihelical secondary structure through the formation of additional (mainly A-U) base-pairs (e.g. an additional approx. 58 A-U base-pairs per molecule of E. coli S-rRNA as judged by u.v. difference spectrophotometry...
摘要
已表明，在0.03 - 1.2M - KCl范围内，Mg2+会影响rRNA的构象。所研究的种类有大肠杆菌的S - rRNA和L - rRNA（分别为较小和较大核糖体亚基颗粒的RNA部分）以及兔的S - rRNA和L - rRNA。2. 在20℃下，于重构缓冲液（0.35M - KCl、0.01M - Tris/HCl，pH7.2）中向rRNA添加Mg2+，通过形成额外的（主要是A - U）碱基对，导致双螺旋二级结构增加（例如，通过紫外差示分光光度法判断，每分子大肠杆菌S - rRNA大约增加58个A - U碱基对……
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A study of the influence of magnesium ions on the conformation of ribosomal ribonucleic acid and on the stability of the larger subribosomal particle of rabbit reticulocytes.镁离子对兔网织红细胞核糖体核糖核酸构象及较大核糖体亚基颗粒稳定性影响的研究。
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