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Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli.通过对在大肠杆菌中表达的基因产物进行功能分析来阐明胡萝卜软腐欧文氏菌类胡萝卜素生物合成途径。
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New functional assignment of the carotenogenic genes crtB and crtE with constructs of these genes from Erwinia species.利用欧文氏菌属物种的类胡萝卜素生成基因crtB和crtE构建体对这些基因进行新的功能分配。
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嗜热栖热菌HB27（一种产生类胡萝卜素色素的嗜热菌）crtB基因的分子克隆及序列分析

Molecular cloning and sequence analysis of the crtB gene of Thermus thermophilus HB27, an extreme thermophile producing carotenoid pigments.

作者信息

Hoshino T, Fujii R, Nakahara T

机构信息

Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.

出版信息

Appl Environ Microbiol. 1993 Sep;59(9):3150-3. doi: 10.1128/aem.59.9.3150-3153.1993.

DOI:10.1128/aem.59.9.3150-3153.1993
PMID:8215386
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC182424/
Abstract

We have cloned and sequenced a 1.5-kb chromosomal fragment of Thermus thermophilus which promoted the overproduction of carotenoids in T. thermophilus. An open reading frame (ORF-A) coding for a polypeptide with 289 amino acids was responsible for carotenoid overproduction. The putative ORF-A protein showed significant homology with the amino acid sequences of crtB gene products (phytoene syntheses) of other microorganisms. The clone containing the ORF-A on a multicopy plasmid produced about three times as much carotenoid as that produced by the host strain, suggesting that the crtB gene product is a rate-limiting enzyme for carotenoid biosynthesis in T. thermophilus.

摘要

我们克隆并测序了嗜热栖热菌的一段1.5kb染色体片段，该片段可促进嗜热栖热菌中类胡萝卜素的过量产生。一个编码含289个氨基酸的多肽的开放阅读框（ORF-A）负责类胡萝卜素的过量产生。推测的ORF-A蛋白与其他微生物的crtB基因产物（八氢番茄红素合成酶）的氨基酸序列具有显著同源性。在多拷贝质粒上含有ORF-A的克隆产生的类胡萝卜素约为宿主菌株产生量的三倍，这表明crtB基因产物是嗜热栖热菌类胡萝卜素生物合成的限速酶。