嗜热栖热菌（Thermus thermophilus）的嘧啶生物合成基因（pyrE和pyrF）
Pyrimidine biosynthesis genes (pyrE and pyrF) of an extreme thermophile, Thermus thermophilus.
作者信息
Yamagishi A, Tanimoto T, Suzuki T, Oshima T
机构信息
Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, Japan.
出版信息
Appl Environ Microbiol. 1996 Jun;62(6):2191-4. doi: 10.1128/aem.62.6.2191-2194.1996.
Abstract
We have isolated uracil auxotrophic mutants of an extreme thermophile, Thermus thermophilus. A part of the pyrimidine biosynthetic operon including genes for orotate phosphoribosyltransferase (pyrE) and for orotidine-5'-monophosphate decarboxylase (pyrF) was cloned and sequenced. The pyrE gene can be a bidirectional marker for the gene manipulation system of the thermophile.
摘要
我们已经分离出嗜热栖热菌（Thermus thermophilus）这种极端嗜热菌的尿嘧啶营养缺陷型突变体。克隆并测序了嘧啶生物合成操纵子的一部分，包括乳清酸磷酸核糖转移酶（pyrE）基因和乳清苷-5'-单磷酸脱羧酶（pyrF）基因。pyrE基因可以作为嗜热菌基因操作系统的双向标记。
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