Gedek B, Kirchgessner M, Wiehler S, Bott A, Eidelsburger U, Roth F X
Institut für Med. Mikrobiologie, Infektions- und Seuchenmedizin, Ludwig-Maximillians-Universität München, Germany.
Arch Tierernahr. 1993;44(3):215-26. doi: 10.1080/17450399309386071.
After a feeding trial of 42 days with 4 x 12 piglets 4 x 6 piglets were slaughtered and the influence of spores of the Bacillus cereus strain FH 1457 S added to the feed on the microorganism counts of Lactobacillus/Bifidobacterium, Eubacteria, Bacteroidaceae, E. coli, Enterococcus and passants (Bacillus cereus) in duodenum, jejunum, ileum, caecum and colon was investigated. Beside a negative control the feed was supplemented with 10(7), 10(8) and 10(9) CFU Bacillus cereus/kg. The feeding trial included two periods each of 21 days. In each period faeces was collected from all animals and the influence on the microorganism counts and also the frequency of resistance of the E. coli and Enterococcus germs against selected antibiotics and chemotherapeutics investigated. The addition of 10(8) CFU Bacillus cereus decreased E. coli counts in duodenum and jejunum, however increased them in ileum, caecum and colon. In the highest dosage the counts of Bacillus cereus in duodenum, caecum, colon and faeces were significantly higher. The addition of Bacillus cereus reduced the counts of E. coli and Enterococcus in faeces in the first period. In the second period the dosage 10(8) CFU showed significant increased counts of E. coli and the sums of microorganisms of the main and satellite flora. The additions had no influence on the frequency of resistance of E. coli and enterococci against the tested antibiotics and synthetic chemotherapeutics. From the addition of Bacillus cereus in this case a selection of factors influencing resistance is not to be expected.
选用4×12头仔猪进行了为期42天的饲养试验，之后屠宰了4×6头仔猪，研究了饲料中添加蜡样芽孢杆菌FH 1457 S菌株的孢子对十二指肠、空肠、回肠、盲肠和结肠中乳酸杆菌/双歧杆菌、真细菌、拟杆菌科、大肠杆菌、肠球菌及过路菌（蜡样芽孢杆菌）数量的影响。除阴性对照外，饲料中分别添加了10⁷、10⁸和10⁹CFU/千克蜡样芽孢杆菌。饲养试验包括两个各为期21天的阶段。在每个阶段，收集所有动物的粪便，研究其对微生物数量的影响，以及大肠杆菌和肠球菌对选定抗生素和化疗药物的耐药频率。添加10⁸CFU蜡样芽孢杆菌可使十二指肠和空肠中的大肠杆菌数量减少，但回肠、盲肠和结肠中的大肠杆菌数量增加。在最高剂量组，十二指肠、盲肠、结肠和粪便中的蜡样芽孢杆菌数量显著更高。在第一阶段，添加蜡样芽孢杆菌可使粪便中的大肠杆菌和肠球菌数量减少。在第二阶段，10⁸CFU剂量组的大肠杆菌数量及主要菌群和卫星菌群的微生物总数显著增加。这些添加物对大肠杆菌和肠球菌对受试抗生素和合成化疗药物的耐药频率没有影响。在这种情况下，预计添加蜡样芽孢杆菌不会产生影响耐药性的选择因素。
