Dolezel S, Edvinsson L, Owman C, Owman T
Cell Tissue Res. 1976 Jun 14;169(2):211-20. doi: 10.1007/BF00214209.
The adrenergic innervation of the juxtaglomerular complex was studied in kidneys from mice, rats, guinea-pigs, rabbits, cats, dogs, pigs, monkeys, and humans using fluorescence histochemistry of neuronal noradrenaline and autoradiography of 3H-noradrenaline. The localization of the nerves was established by phase contrast optics or by perfusing the vascular system with India ink. Adrenergic nerve terminals, exhibiting a formaldehyde-induced fluorescence and having the ability to take up and accumulate 3H-noradrenaline, were easily identified when they enclosed the glomerular afferent arteriole. They continued in between and close to the macula densa and lacis cells to supply the glomerular efferent arteriole. The nerves could be seen to accompany this arteriole for a considerable distance until they branched off to the vasa recta in the juxtamedullary region and to adjacent cortical veins. This innervation pattern was found to be a constant feature except in kidneys from guinea-pigs and cats, in which post-glomerular units. The fluorescence in all adrenergic fibres supplying the juxtaglomerular complex disappeared after removal of the aortico-renal ganglion, showing that they belong to a common system of renal sympathetic nerves.
利用神经元去甲肾上腺素的荧光组织化学和3H-去甲肾上腺素放射自显影技术，对小鼠、大鼠、豚鼠、兔子、猫、狗、猪、猴子和人类肾脏中的肾小球旁复合体的肾上腺素能神经支配进行了研究。通过相差光学显微镜或用印度墨汁灌注血管系统来确定神经的定位。当肾上腺素能神经末梢围绕肾小球入球小动脉时，很容易识别出它们，这些末梢呈现甲醛诱导的荧光，并具有摄取和积累3H-去甲肾上腺素的能力。它们在致密斑和球外系膜细胞之间并靠近这些细胞继续延伸，以供应肾小球出球小动脉。可以看到这些神经伴随该小动脉走行相当长的距离，直到它们分支进入髓质旁区域的直小血管和相邻的皮质静脉。除了豚鼠和猫的肾脏外，这种神经支配模式是一个恒定的特征，在豚鼠和猫的肾脏中，肾小球后单位。切除主动脉-肾神经节后，供应肾小球旁复合体的所有肾上腺素能纤维的荧光消失，表明它们属于肾交感神经的一个共同系统。
