小鼠骨髓瘤细胞的体细胞杂交

Somatic cell hybridization of mouse myeloma cells.

作者信息

Margulies D H, Kuehl W M, Scharff M D

出版信息

Cell. 1976 Jul;8(3):405-15. doi: 10.1016/0092-8674(76)90153-7.

Abstract

Somatic cell hybrides between different mouse myeloma cell lines have been readili isolated using modifications of existing techniques. The hybrid nature of these cells was established by HAT or HAT-ouabain selective procedures, their chromosome number, and, in one case, H-2 surface antifen expression. Three hybrid cell lines are described here in detail: an IgG2B, K X LgG2a, k; an IgG1, k X IgG2b, k; and an IgG1, k X IgM, Lambda. In all cases, both parental types of H and L chains are expressed in the hybrid cells and no new chains are observed. However, molecules possessing disulfide-bonded mixtures of parental H and/or L chains are seen. Analysis of subclones of these hybrids indicates considerable stability in the expression of the immunoglobulins for up to 13 months. However, segregant clones no longer synthesizing one or more of the parental H or L chains arise frequently.

摘要

通过对现有技术进行改进，已轻松分离出不同小鼠骨髓瘤细胞系之间的体细胞杂种。这些细胞的杂种性质通过HAT或HAT-哇巴因选择程序、它们的染色体数目以及在一个案例中的H-2表面抗原表达得以确定。这里详细描述了三种杂种细胞系：一种IgG2B、κ×IgG2a、κ；一种IgG1、κ×IgG2b、κ；以及一种IgG1、κ×IgM、λ。在所有情况下，亲本类型的重链和轻链在杂种细胞中均有表达，且未观察到新的链。然而，可见具有亲本重链和/或轻链二硫键结合混合物的分子。对这些杂种的亚克隆分析表明，免疫球蛋白的表达在长达13个月的时间里具有相当的稳定性。然而，经常会出现不再合成一种或多种亲本重链或轻链的分离克隆。