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导入小鼠淋巴细胞系的免疫球蛋白κ基因的调控表达。

Regulated expression of an immunoglobulin kappa gene introduced into a mouse lymphoid cell line.

作者信息

Rice D, Baltimore D

出版信息

Proc Natl Acad Sci U S A. 1982 Dec;79(24):7862-5. doi: 10.1073/pnas.79.24.7862.

DOI:10.1073/pnas.79.24.7862
PMID:6818555
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC347449/
Abstract

We have introduced a functionally rearranged murine kappa light chain immunoglobulin (Ig) gene into an Abelson murine leukemia virus-transformed lymphoid cell line. Plasmid pSV2gpt-kappa 41, containing the kappa light chain gene from the myeloma MOPC41 and the selectable marker gene gpt, was introduced into 81A-2 cells by the calcium phosphate coprecipitation technique. Cells expressing the gpt gene were selected by growth in medium containing mycophenolic acid. One transfected cell line, kappa-2, was shown to make kappa mRNA and polypeptide chains and to assemble the kappa chain product with gamma 2b heavy chains to form an apparently complete IgG2b. When bacterial lipopolysaccharide was added to the growth medium, levels of kappa mRNA and polypeptide increased, showing regulated expression of the introduced kappa gene.

摘要

我们已将功能重排的小鼠κ轻链免疫球蛋白（Ig）基因导入阿贝尔逊小鼠白血病病毒转化的淋巴样细胞系。通过磷酸钙共沉淀技术，将含有来自骨髓瘤MOPC41的κ轻链基因和选择标记基因gpt的质粒pSV2gpt - κ41导入81A - 2细胞。通过在含有霉酚酸的培养基中生长来选择表达gpt基因的细胞。一个转染细胞系κ - 2被证明能产生κ mRNA和多肽链，并将κ链产物与γ2b重链组装形成明显完整的IgG2b。当向生长培养基中添加细菌脂多糖时，κ mRNA和多肽的水平增加，表明导入的κ基因表达受到调控。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f7e/347449/d651e82d400f/pnas00463-0262-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f7e/347449/3977cc7097d9/pnas00463-0261-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f7e/347449/c05a83f59a05/pnas00463-0262-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f7e/347449/d651e82d400f/pnas00463-0262-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f7e/347449/3977cc7097d9/pnas00463-0261-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f7e/347449/c05a83f59a05/pnas00463-0262-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f7e/347449/d651e82d400f/pnas00463-0262-b.jpg

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本文引用的文献

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