Wittenberg R H, Willburger R E, Kleemeyer K S, Peskar B A
Department of Orthopaedic Surgery, Ruhr University, Bochum, Germany.
Arthritis Rheum. 1993 Oct;36(10):1444-50. doi: 10.1002/art.1780361017.
To determine the major source of eicosanoid release in arthritic joint tissues and to examine the modulation of this release by indomethacin and diclofenac.
Release of prostaglandin E2 (PGE2), 6-keto-PGF1 alpha, leukotriene B4 (LTB4), and LTC4 was measured in supernatants of synovial tissue, cartilage, and bone incubates from patients with osteoarthritis, active rheumatoid arthritis (RA), inactive RA, and pseudogout. Radioimmunoassay (RIA) was used to determine the levels of the eicosanoids.
Addition of the divalent cation ionophore A23187 resulted in significant release of all eicosanoids measured from synovial tissue, but not from cartilage, cortical bone, or cancellous bone. PG release was significantly inhibited by the addition of indomethacin or diclofenac at either 10(-5) moles/liter or 10(-7) moles/liter. The amount of LTC4 released from cartilage and bone was only slightly above the detection limit of the RIA, whereas large amounts were released from synovial tissue. Neither indomethacin nor diclofenac had an effect on LTC4 release. LTC4 release from synovial tissue of patients with inactive RA was significantly decreased in comparison with the levels from synovial tissue of patients with the other joint diseases. There was no significant difference in PG release among patients in the various disease groups.
Synovial tissue appears to be the major source of eicosanoids in synovial fluid. Indomethacin and diclofenac inhibit the release of PG, but not LT, from various joint tissues.
确定关节炎关节组织中类花生酸释放的主要来源，并研究吲哚美辛和双氯芬酸对这种释放的调节作用。
测定骨关节炎、活动期类风湿关节炎（RA）、非活动期RA和假性痛风患者滑膜组织、软骨和骨培养上清液中前列腺素E2（PGE2）、6-酮-前列腺素F1α、白三烯B4（LTB4）和LTC4的释放量。采用放射免疫分析法（RIA）测定类花生酸的水平。
添加二价阳离子载体A23187可导致滑膜组织中所有测定的类花生酸显著释放，但软骨、皮质骨或松质骨中则无此现象。在10⁻⁵摩尔/升或10⁻⁷摩尔/升浓度下添加吲哚美辛或双氯芬酸可显著抑制PG释放。从软骨和骨中释放的LTC4量仅略高于RIA的检测限，而滑膜组织中则大量释放。吲哚美辛和双氯芬酸对LTC4释放均无影响。与其他关节疾病患者的滑膜组织水平相比，非活动期RA患者滑膜组织中LTC4的释放显著降低。不同疾病组患者之间的PG释放无显著差异。
滑膜组织似乎是滑液中类花生酸的主要来源。吲哚美辛和双氯芬酸可抑制各种关节组织中PG的释放，但不能抑制LT的释放。
