Lippiello L, Yamamoto K, Robinson D, Mankin H J
Arthritis Rheum. 1978 Nov-Dec;21(8):909-17. doi: 10.1002/art.1780210807.
Short term incubations of articular cartilage in media from cultures of rheumatoid synovial tissue result in marked inhibition of 3H-glycine and Na235SO4 incorporation into trichloroacetic acid (TCA) insoluble macromolecules by the cartilage segments with little effect on degradation of prelabeled cartilage matrix. This inhibition is time dependent and not observed in cartilage segments incubated in media derived from rheumatoid synovia cultured in the presence of indomethacin (a specific inhibitor of prostaglandin synthesis). In addition, no indomethacin-sensitive cartilage inhibitory activity was detectable in media from cultures of normal synovia. The ether solubility of the majority of the inhibitory activity and the indomethacin sensitivity suggest that the inhibitor(s) are prostaglandins.
类风湿性滑膜组织培养物的培养基中关节软骨的短期孵育导致软骨片段对3H-甘氨酸和Na235SO4掺入三氯乙酸（TCA）不溶性大分子的显著抑制，而对预标记的软骨基质降解影响很小。这种抑制是时间依赖性的，在吲哚美辛（前列腺素合成的特异性抑制剂）存在下培养的类风湿性滑膜培养基中孵育的软骨片段中未观察到。此外，在正常滑膜培养物的培养基中未检测到对吲哚美辛敏感的软骨抑制活性。大多数抑制活性的醚溶性和对吲哚美辛的敏感性表明抑制剂是前列腺素。
