Usawattanakul W, Kitikoon V, Chaicumpa W, Janecharut T, Wongwit W, Pattara-Arechachai J, Tharavanij S
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Asian Pac J Allergy Immunol. 1993 Jun;11(1):71-7.
Protective efficacy of the extracts of cercariae, schistosomulae and adult worms of S. mekongi was studied in mice receiving immunizations with these extracts emulsified with Freund's complete adjuvant initially and incomplete adjuvant subsequently, and compared with mice receiving physiological saline with or without adjuvants as controls. After challenge with cercariae, the animals were sacrificed and the larvae or adult worms harvested by lung recovery and perfusion techniques on day 5 and weeks 6-8, respectively. Worm reduction rates were significantly higher in mice receiving extracts of schistosomula (59%) and adult worms (51%) than in those receiving the cercarial extracts (31%). Similar findings were obtained with the perfusion technique showing worm reduction rates of 57%, 53% and 30% in mice receiving extracts of schistosomulae, adult worms and cercariae, respectively. ELISA antibody titers were correspondingly increased in mice receiving extracts of schistosomulae and adult worms, but not in those receiving cercariae. This apparent association may be inadequate to suggest that the increase in ELISA titer be used as an indicator for resistance in mekongi schistosomiasis.
在小鼠中研究了湄公血吸虫尾蚴、童虫和成虫提取物的保护效力。最初用弗氏完全佐剂乳化这些提取物对小鼠进行免疫，随后用不完全佐剂进行免疫，并与接受含或不含佐剂生理盐水的小鼠作为对照进行比较。在用尾蚴攻击后，分别在第5天和第6 - 8周通过肺回收和灌注技术处死动物并收集幼虫或成虫。接受童虫提取物（59%）和成虫提取物（51%）的小鼠的虫体减少率显著高于接受尾蚴提取物的小鼠（31%）。灌注技术也得到了类似的结果，接受童虫、成虫和尾蚴提取物的小鼠的虫体减少率分别为57%、53%和30%。接受童虫和成虫提取物的小鼠的ELISA抗体滴度相应升高，但接受尾蚴提取物的小鼠则未升高。这种明显的关联可能不足以表明ELISA滴度的升高可用作湄公血吸虫病抗性的指标。
