Janecharut T, Kitikoon V, Usawattanakul W, Sornmani S
Department of Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 1988 Mar;19(1):123-9.
An investigation on immunity induced by Schistosoma spindale cercariae (cattle and swamp buffalo schistosome) against S. mekongi (human schistosome) was conducted in Swiss albino mice. The studies comprised the development patterns of homologous immunity of S. spindale and heterologous immunity induced by S. spindale against S. mekongi. The development pattern of homologous immunity was studied in mice with an immunization of 100 S. spindale cercariae. At one week intervals, between 2 to 16 weeks after immunization, they were each challenged with 500 S. spindale cercariae. Significant homologous immunity, as judged by lung recovery assay five days after challenge, occurred from week 5 to week 16 with week 8 giving the highest homologous immunity (68.1% of schistosomular reduction). Using the above information mice, with an eight-week immunization period of 100 S. spindale cercariae, were tested for resistance to heterologous S. mekongi infection. The criteria used to evaluate their immune status was schistosomular lung recovery, daily egg output, worm recovery and tissue egg count. The results showed that mice immunized with S. spindale cercariae could develop heterologous immunity against S. mekongi infection. Manifestation of immunity was demonstrated by significant reduction in mean schistosomular recovery (31.4%), in mean daily egg output per female worm (16.7%), in mean worm recovery (64.2%) and in mean egg deposition in the liver tissue and intestines per female worm (37.05%).
在瑞士白化小鼠中开展了一项关于纺锤血吸虫（牛和沼泽水牛血吸虫）尾蚴诱导的针对湄公血吸虫（人血吸虫）的免疫研究。这些研究包括纺锤血吸虫的同源免疫以及纺锤血吸虫诱导的针对湄公血吸虫的异源免疫的发展模式。通过用100条纺锤血吸虫尾蚴免疫小鼠来研究同源免疫的发展模式。在免疫后2至16周期间，每隔一周用500条纺锤血吸虫尾蚴对它们进行攻击。根据攻击后五天的肺部回收率测定，从第5周开始到第16周出现了显著的同源免疫，其中第8周的同源免疫最强（血吸虫幼虫减少68.1%）。利用上述信息，用100条纺锤血吸虫尾蚴进行为期八周免疫的小鼠，接受了针对异源湄公血吸虫感染的抗性测试。用于评估其免疫状态的标准是血吸虫幼虫肺部回收率、每日产卵量、虫体回收率和组织虫卵计数。结果表明，用纺锤血吸虫尾蚴免疫的小鼠可对湄公血吸虫感染产生异源免疫。免疫表现为平均血吸虫幼虫回收率显著降低（31.4%）、每条雌虫平均每日产卵量显著降低（16.7%）、平均虫体回收率显著降低（64.2%）以及每条雌虫在肝脏组织和肠道中的平均虫卵沉积显著降低（37.05%）。
