[核糖体核糖核蛋白前体颗粒在甲泛影酰胺梯度中的等密度离心]
[Isopycnic centrifugation in metrizanide gradient of ribosomal ribonucleoprotein precursor particles].
作者信息
Auger M A, Longuet M
出版信息
C R Acad Hebd Seances Acad Sci D. 1976 May 31;282(21):1889-92.
PMID:821661
Abstract
Metrizamide has been described as a new medium for isopycnic centrifugation of unfixed biological particles. In the murine leukemia cell line L 5178 Y, the buoyant density of 80 S and 60 S preribosomal particles was found to be 1,22 g/cm3, which is noticeably lighter than buoyant densities of mature ribosomal particles.
摘要
甲泛葡胺已被描述为一种用于未固定生物颗粒等密度离心的新型介质。在小鼠白血病细胞系L 5178 Y中，发现80 S和60 S前核糖体颗粒的浮力密度为1.22 g/cm³，明显低于成熟核糖体颗粒的浮力密度。
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