Suwalsky M, Frías J
Faculty of Chemical Sciences, University of Concepción, Chile.
Z Naturforsch C J Biosci. 1993 Jul-Aug;48(7-8):632-9. doi: 10.1515/znc-1993-7-817.
This study deals with the structural perturbations that the aminoglycoside antibiotic gentamicin (GENT) can produce to phospholipid bilayers. Two multi-bilayer systems, one built-up of dimyristoylphosphatidylcholine (DMPC) and the other of dimyristoylphosphatidylethanolamine (DMPE) were allowed to interact with GENT. The experiments were performed in both a hydrophobic and a hydrophilic medium below the phospholipid main transition temperatures. X-ray diffraction techniques were used to determine the extent of the perturbation induced by GENT. The maximum effect was attained when GENT interacted with DMPC in the hydrophobic medium. On the other hand, GENT in aqueous solutions was unable to perturb in any significant extent the structure of the phospholipids under study.
本研究探讨了氨基糖苷类抗生素庆大霉素（GENT）对磷脂双层膜可能产生的结构扰动。使用了两个多层膜系统，一个由二肉豆蔻酰磷脂酰胆碱（DMPC）构成，另一个由二肉豆蔻酰磷脂酰乙醇胺（DMPE）构成，并使其与GENT相互作用。实验在低于磷脂主要转变温度的疏水和亲水介质中进行。采用X射线衍射技术来确定GENT引起的扰动程度。当GENT在疏水介质中与DMPC相互作用时，达到了最大效果。另一方面，水溶液中的GENT在任何显著程度上都无法扰动所研究的磷脂结构。
