牛对亲和纯化的牛疱疹病毒1型抗独特型抗体和糖蛋白的免疫反应比较。

Comparison of bovine immune responses to affinity-purified bovine herpesvirus-1 antiidiotypes and glycoproteins.

作者信息

Orten D J, Xue W, van Drunen Littel-van den hurk S, Abdelmagid O Y, Reddy D N, Campos M, Babiuk L A, Blecha F, Minocha H C

机构信息

Department of Pathology and Microbiology, Kansas State University, Manhattan.

出版信息

Viral Immunol. 1993 Summer;6(2):109-17. doi: 10.1089/vim.1993.6.109.

DOI:10.1089/vim.1993.6.109

PMID:8216712

Abstract

Bovine immune responses to rabbit antiidiotypic antibodies (anti-Id) against neutralizing monoclonal antibodies to bovine herpesvirus-1 (BHV-1) envelope glycoproteins and to BHV-1 glycoproteins were compared. Glycoprotein-immunized animals produced high titers of anti-BHV-1 antibodies and were protected against BHV-1 challenge. Recombinant bovine interleukin-2 (rIL-2)-treated, anti-Id-immunized animals showed a slight reduction in clinical disease, and one calf produced BHV-1-neutralizing antibodies. Treatment with rIL-2 augmented non-BHV-1-specific immune responses. However, even with rIL-2 as an adjuvant, the mixture of polyclonal anti-Id did not elicit a consistent, protective BHV-1-specific immune response in calves.

摘要

比较了牛对兔抗独特型抗体（抗Id）的免疫反应，这些抗Id是针对牛疱疹病毒1型（BHV - 1）包膜糖蛋白的中和单克隆抗体以及针对BHV - 1糖蛋白的。用糖蛋白免疫的动物产生了高滴度的抗BHV - 1抗体，并对BHV - 1攻击具有抵抗力。经重组牛白细胞介素 - 2（rIL - 2）处理的抗Id免疫动物临床疾病略有减轻，且有一头小牛产生了BHV - 1中和抗体。rIL - 2处理增强了非BHV - 1特异性免疫反应。然而，即使将rIL - 2用作佐剂，多克隆抗Id混合物在小牛中也未引发一致的、具有保护性的BHV - 1特异性免疫反应。