Israel B A, Marshall R L, Letchworth G J
Department of Veterinary Science, University of Wisconsin, Madison 53706.
Vaccine. 1988 Aug;6(4):349-56. doi: 10.1016/0264-410x(88)90182-x.
Bovine herpesvirus-1 (BHV-1) envelope glycoproteins gI, gIII and gIV were individually purified on monoclonal antibody affinity columns and injected intradermally into BHV-1 seronegative calves. The calves developed serum neutralizing antibodies that monospecifically precipitated the immunizing glycoprotein from a preparation of 125I-labelled BHV-1 envelope proteins. A competitive radioimmunoassay using the bovine antisera demonstrated that known functional epitopes had been retained in the glycoprotein vaccines. Calves immunized with the gI, gIII or gIV glycoproteins were not protected from intranasal challenge with BHV-1 and had levels and duration of viral shedding in their nasal secretions similar to those of non-immunized control calves.
牛疱疹病毒1型（BHV-1）包膜糖蛋白gI、gIII和gIV分别在单克隆抗体亲和柱上纯化，并皮内注射到BHV-1血清阴性的犊牛体内。这些犊牛产生了血清中和抗体，该抗体能从125I标记的BHV-1包膜蛋白制剂中特异性沉淀免疫糖蛋白。使用牛抗血清的竞争性放射免疫测定表明，已知的功能表位保留在糖蛋白疫苗中。用gI、gIII或gIV糖蛋白免疫的犊牛不能抵御BHV-1的鼻内攻击，其鼻分泌物中的病毒排出水平和持续时间与未免疫的对照犊牛相似。
