Runting A S, Stacker S A, Wilks A F
Ludwig Institute for Cancer Research, Post Office Royal Melbourne Hospital, Parkville, Victoria, Australia.
Growth Factors. 1993;9(2):99-105.
The cDNA of a novel mouse cell surface receptor (tie2) has been isolated from a mouse lung library. The predicted amino acid sequence of tie2 encodes a protein of 1122 amino acids, with an extracellular domain and an intracellular tyrosine kinase domain bisected by a transmembrane region. The extracellular domain consists of two Ig-like domains, three cysteine-rich domains and three fibronectin type III repeats whilst the intracellular tyrosine kinase domain has a short insert region of 15 amino acids. In vitro transcription/translation of the tie2 cDNA demonstrates that it encodes a glycoprotein of some 145 kDa. The tie2 protein exhibits a high degree of similarity to the cell surface receptor tie, (Partanen, J. et al., (1992) Mol. Cell. Biol., 12, 1698-1707), and together with this protein defines a new class of cell surface receptor.
一种新型小鼠细胞表面受体（tie2）的互补DNA（cDNA）已从小鼠肺文库中分离出来。tie2预测的氨基酸序列编码一个由1122个氨基酸组成的蛋白质，其细胞外结构域和细胞内酪氨酸激酶结构域被一个跨膜区域一分为二。细胞外结构域由两个免疫球蛋白（Ig）样结构域、三个富含半胱氨酸的结构域和三个纤连蛋白III型重复序列组成，而细胞内酪氨酸激酶结构域有一个由15个氨基酸组成的短插入区域。tie2 cDNA的体外转录/翻译表明它编码一种约145 kDa的糖蛋白。tie2蛋白与细胞表面受体tie表现出高度相似性（帕尔塔宁，J.等人，（1992年）《分子与细胞生物学》，12卷，1698 - 1707页），并且与该蛋白共同定义了一类新的细胞表面受体。
