Iotti S, Lodi R, Frassineti C, Zaniol P, Barbiroli B
Istituto di Patologia Medica D. Campanacci, Università di Bologna, Italy.
NMR Biomed. 1993 Jul-Aug;6(4):248-53. doi: 10.1002/nbm.1940060404.
In this study we compared the kinetics of phosphocreatine (PCr) and P(i) recovery, and their dependency on cytosolic pH in 38 normal individuals. Spectra were acquired during rest, work and recovery. A time resolution of 10 s was used to obtain detailed information. The kinetics of PCr and P(i) recovery almost overlapped when the lowest value of cytosolic pH reached during recovery (termed the minimum pH) was < 6.95, while they were completely dissociated when the minimum pH was > 6.95. This result is interpreted as indirect in vivo evidence of the kinetic control exerted by ADP on mitochondrial oxidation. Our results represent a rationale for new experimental conditions to be used in clinical routine studies of pathologies due to primary or secondary mitochondrial malfunction.
在本研究中，我们比较了38名正常个体中磷酸肌酸（PCr）和无机磷（P(i)）的恢复动力学，以及它们对细胞溶质pH的依赖性。在静息、运动和恢复过程中采集光谱。采用10秒的时间分辨率以获取详细信息。当恢复过程中达到的细胞溶质pH最低值（称为最低pH）<6.95时，PCr和P(i)的恢复动力学几乎重叠，而当最低pH>6.95时，它们则完全分离。该结果被解释为ADP对线粒体氧化进行动力学控制的间接体内证据。我们的结果为原发性或继发性线粒体功能障碍所致疾病的临床常规研究中使用新的实验条件提供了理论依据。
