Yamaguchi A, Urano T, Fushida S, Furukawa K, Nishimura G, Yonemura Y, Miyazaki I, Nakagawara G, Shiku H
First Department of Surgery, Fukui Medical School, Japan.
Br J Cancer. 1993 Nov;68(5):1020-4. doi: 10.1038/bjc.1993.473.
The expression of nm23-H1 mRNA and protein was studied in colorectal cancers by Northern blotting and immunohistochemistry. All 21 colorectal cancers studied by Northern blotting had increased levels of nm23-H1 mRNA relative to the adjacent normal colonic mucosa. Increased nm23-H1 protein expression was also observed in all 36 colorectal cancer cases including those studied by Northern blotting. There was no significant correlation between nm23-H1 expression and tumour histology, serosal invasion, lymphatic invasion, venous invasion, or lymph node metastasis. However, the expression of both mRNA and protein was significantly lower in tumours associated with liver metastasis than in those without such metastasis. These observations indicate that the nm23 gene may play a role in the suppression of liver metastasis of colorectal cancer.
通过Northern印迹法和免疫组织化学研究了nm23-H1 mRNA和蛋白在结直肠癌中的表达。通过Northern印迹法研究的所有21例结直肠癌相对于相邻正常结肠黏膜，nm23-H1 mRNA水平均升高。在包括通过Northern印迹法研究的所有36例结直肠癌病例中也观察到nm23-H1蛋白表达增加。nm23-H1表达与肿瘤组织学、浆膜侵犯、淋巴侵犯、静脉侵犯或淋巴结转移之间无显著相关性。然而，与肝转移相关的肿瘤中mRNA和蛋白的表达均显著低于无肝转移的肿瘤。这些观察结果表明nm23基因可能在抑制结直肠癌肝转移中起作用。
