Webster R, Pulford K, Jones M, Micklem K, de Waele M, Mayne K, Tse A, Mason D Y
Department of Haematology, John Radcliffe Hospital, Oxford.
Br J Haematol. 1993 Aug;84(4):602-7. doi: 10.1111/j.1365-2141.1993.tb03134.x.
Several monoclonal antibodies have been raised in the past which react with human plasma cells but they have all shown additional reactions with other cell types. In this paper we describe a new monoclonal antibody, ZB51, which recognizes an intracellular antigen in normal plasma cells in cryostat tissue sections and cell smears, and which also reacts with neoplastic cells in most cases of myeloma and with plasma cell lines. The antibody shows minimal reactivity with a few eosinophils and myelocytes in bone marrow and stains a myeloid cell line. Whilst normal epithelium is not labelled, antibody ZB51 stains two carcinoma cell lines. Although it was not possible to characterize the target antigen in terms of molecular weight, the reactivity of antibody ZB51 with normal and neoplastic plasma cells makes it a useful new immunocytochemical reagent.
过去已产生了几种与人类浆细胞发生反应的单克隆抗体，但它们均显示出与其他细胞类型的额外反应。在本文中，我们描述了一种新的单克隆抗体ZB51，它能识别冷冻切片组织和细胞涂片的正常浆细胞中的一种细胞内抗原，并且在大多数骨髓瘤病例中也与肿瘤细胞以及浆细胞系发生反应。该抗体与骨髓中的少数嗜酸性粒细胞和髓细胞反应性极低，并能对一种髓系细胞系进行染色。虽然正常上皮未被标记，但抗体ZB51能对两种癌细胞系进行染色。尽管无法根据分子量对靶抗原进行表征，但抗体ZB51与正常和肿瘤性浆细胞的反应性使其成为一种有用的新型免疫细胞化学试剂。
