Fike R M, van Oss C J
In Vitro. 1976 Jun;12(6):428-36. doi: 10.1007/BF02806022.
By measuring the electro-osmotic flow velocity of buffer through a glass capillary, on the inner surface of which cells have been grown, the zota-protential of these cells can be determined without the changes in zota-potential, viability and morphology caused by the chemical, enzymatic or mechanical pretreatment hitherto necessary for cell dispersion in the microelectrophoretic method. Cells grown in a monolayer inside the capillaries were: BGM, HEP-2, and RPMI 1846 (the last one also could be grown in suspension).
通过测量缓冲液在已生长细胞的玻璃毛细管内的电渗流速度，可以确定这些细胞的ζ电位，而不会出现微电泳法中迄今为止细胞分散所需的化学、酶促或机械预处理所导致的ζ电位、活力和形态变化。在毛细管内单层生长的细胞有：BGM、HEP-2和RPMI 1846（最后一种也可以悬浮生长）。
