Buggé C J, Tucker M D, Garcia D B, Kvalo L T, Wilhelm J A
CEDRA Corporation, Austin, TX 78754.
J Pharm Biomed Anal. 1993 Sep;11(9):809-15. doi: 10.1016/0731-7085(93)80073-a.
A method for the quantitation of mirfentanil hydrochloride (A-3508.HCl) in human plasma is presented for the first time, using LC-MS with single ion monitoring. The drug is extracted with a C-18 solid-phase cartridge and the extract is analysed using a 3 cm C-18 column connected to the ion source of a mass spectrometer via a thermospray interface. The intense ion produced by the protonated molecular ion at m/z 377 is detected by the mass spectrometer in positive-ion mode. The range of quantitation is 0.4-100 ng ml-1 from a 0.5 ml plasma sample. Results of assay validation are given. The method was used to analyse samples from a human pharmacokinetic study following intravenous administration of mirfentanil hydrochloride.
首次提出了一种使用液相色谱-质谱联用单离子监测法测定人血浆中盐酸米芬太尼（A-3508.HCl）的方法。该药物用C-18固相萃取柱萃取，萃取物通过热喷雾接口连接到质谱仪离子源的3 cm C-18柱进行分析。质谱仪在正离子模式下检测到质子化分子离子m/z 377产生的强离子。从0.5 ml血浆样品中定量的范围是0.4 - 100 ng/ml。给出了分析方法验证的结果。该方法用于分析静脉注射盐酸米芬太尼后人药代动力学研究的样品。
