Kulakovskaia T V, Kuranov A Iu, Okorokov L A
Biokhimiia. 1993 Aug;58(9):1389-93.
Heparin (20-40 micrograms/ml) inhibits by 50% both the ATP hydrolase and the delta pH formation in isolated yeast vacuoles. The GTPase activity is not inhibited under these conditions. ATP prevents this inhibition, being added 10 min before heparin. Heparin suppresses the phosphorylation of vacuolar proteins in vitro. It is supposed that the protein kinase, similar to casein kinase I, participates in the regulation of activity of the vacuolar ATPase.
肝素（20 - 40微克/毫升）可使分离出的酵母液泡中的ATP水解酶活性和ΔpH形成均受到50%的抑制。在这些条件下，GTP酶活性未受抑制。在加入肝素前10分钟添加ATP可防止这种抑制作用。肝素在体外可抑制液泡蛋白的磷酸化。据推测，类似于酪蛋白激酶I的蛋白激酶参与了液泡ATP酶活性的调节。
