Renda T, Negri L, Tooyama I, Casu C, Melchiorri P
Institute of Human Anatomy, University La Sapienza, Rome, Italy.
Neuroreport. 1993 Sep 3;4(10):1143-6.
Previous biochemical and pharmacological studies have shown that [D-Ala2]-deltorphin-I (DADTI) has a high affinity and selectivity for delta-opioid receptors. In this study, designed to provide morphological details, the distribution of DADTI binding sites was examined by autoradiography on coronal, sagittal and horizontal frozen sections of adult rat brain. The sections were incubated with tritiated DADTI solution and exposed for 12 weeks to a 3H-sensitive film. DADTI labelling clearly demonstrated selective and high affinity binding sites of delta-opioid type in several brain regions, including olfactory system, neostriatum, nucleus accumbens, and cortical layers I-II and V-VI.
先前的生物化学和药理学研究表明，[D-丙氨酸2]-强啡肽-I（DADTI）对δ阿片受体具有高亲和力和选择性。在本旨在提供形态学细节的研究中，通过放射自显影术在成年大鼠脑的冠状、矢状和水平冰冻切片上检查了DADTI结合位点的分布。切片与氚标记的DADTI溶液孵育，并在3H敏感胶片上曝光12周。DADTI标记清楚地显示了δ阿片类受体在几个脑区的选择性和高亲和力结合位点，包括嗅觉系统、新纹状体、伏隔核以及皮质I-II层和V-VI层。
