Tooyama I, Abe H, Renda T, Erspamer V, Kimura H
Institute of Molecular Neurobiology, Shiga University of Medical Science, Otsu, Japan.
Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9635-9. doi: 10.1073/pnas.90.20.9635.
Using a specific antiserum recently raised against [D-Ala2]deltorphin I (DADTI: Tyr-D-Ala-Phe-Asp-Val-Val-Gly-NH2), a highly selective ligand for delta-opioid receptors, we have previously demonstrated the occurrence of positive immunostaining in several structures of mouse brain. We describe here the neuroanatomical distribution patterns of DADTI-immunoreactive neuronal bodies, axons, and tanycytes in rat brain. Positive neuronal somata were localized mainly in the ventral mesencephalon, including the ventral tegmental area and the pars compacta of the substantia nigra. A minor population of positive somata was found in the pars reticulata and pars lateralis of the substantia nigra, raphe nuclei, supramammillary nucleus, and retrorubral reticular nucleus. All these regions, except for the supramammillary nucleus, contain dopamine cell bodies. Intensely stained positive nerve fibers could be traced along the medial forebrain bundle. Dense positive terminals were seen in the neostriatum, nucleus accumbens shell, olfactory tubercle, septal areas, cingulate, and medial prefrontal cortex. Double-immunostaining study revealed that, in the substantia nigra, almost all (97.8%) DADTI-positive neurons colocalized with tyrosine hydroxylase (TH), and the doubly stained cells occupied about one-third (29.1%) of the total population of TH-positive neurons. Only a few DADTI/TH-positive cells also stained for 28-kDa calbindin D, although many neurons double-stained for 28-kDa calbindin D and TH. In contrast, the supramammillary nucleus contained a number of DADTI-positive cells, which nearly always stained positively for 28-kDa calbindin D but did not stain for TH. The association of DADTI-like immunoreactivity with certain dopaminergic pathways seems of particular interest. A small population of DADTI-immunostained tanycytes was present in the ventral part of the third ventricle wall.
我们最近制备了一种针对[D - Ala2]强啡肽I（DADTI：Tyr - D - Ala - Phe - Asp - Val - Val - Gly - NH2，一种δ阿片受体的高度选择性配体）的特异性抗血清，此前我们已证实在小鼠脑的多个结构中存在阳性免疫染色。在此，我们描述大鼠脑中DADTI免疫反应性神经元胞体、轴突和伸长细胞的神经解剖分布模式。阳性神经元胞体主要定位于中脑腹侧，包括腹侧被盖区和黑质致密部。在黑质网状部、黑质外侧部、中缝核、乳头体上核和红核后网状核中发现少量阳性胞体。除乳头体上核外，所有这些区域都含有多巴胺细胞体。沿内侧前脑束可追踪到强阳性染色的神经纤维。在新纹状体、伏隔核壳、嗅结节、隔区、扣带回和内侧前额叶皮质可见密集的阳性终末。双重免疫染色研究显示，在黑质中，几乎所有（97.8%）DADTI阳性神经元与酪氨酸羟化酶（TH）共定位，双染细胞约占TH阳性神经元总数的三分之一（29.1%）。只有少数DADTI/TH阳性细胞也对28 kDa钙结合蛋白D染色，尽管许多神经元对28 kDa钙结合蛋白D和TH双染。相比之下，乳头体上核含有许多DADTI阳性细胞，这些细胞几乎总是对28 kDa钙结合蛋白D呈阳性染色，但对TH不染色。DADTI样免疫反应性与某些多巴胺能通路的关联似乎特别令人感兴趣。在第三脑室壁腹侧存在少量DADTI免疫染色的伸长细胞。
