Platt B, Haas H, Büsselberg D
Heinrich Heine Universität, Düsseldorf, Germany.
Neuroreport. 1993 Sep 10;4(11):1251-4. doi: 10.1097/00001756-199309000-00010.
The pH-dependence of aluminium (Al) blockade of voltage activated calcium channels (VACCs) was investigated. Using cultures of rat dorsal root ganglion (DRG) neurones, whole-cell patch-clamp experiments were performed. Various concentrations of Al were extracellularly applied within solutions of different pH-values. The block of VACC currents was highly pH-dependent. At pH 7.3-7.8, the concentration-response curve shifted slightly to higher concentrations, whereas at pH 6.4-6.9 a pronounced shift to lower concentrations was observed. This effect could be due to changes of the chemical equilibria of the different Al species or to altered properties of the VACCs. Thus, pH-shifts may influence the interactions of Al with VACCs making them more susceptible to the effects of Al and therefore contribute to its toxicity.
研究了铝（Al）对电压激活钙通道（VACC）的阻断作用与pH值的关系。使用大鼠背根神经节（DRG）神经元培养物进行全细胞膜片钳实验。在不同pH值的溶液中细胞外施加各种浓度的Al。VACC电流的阻断高度依赖于pH值。在pH 7.3 - 7.8时，浓度 - 反应曲线略微向更高浓度偏移，而在pH 6.4 - 6.9时，观察到明显向更低浓度偏移。这种效应可能是由于不同铝物种的化学平衡变化或VACC特性改变所致。因此，pH值变化可能影响Al与VACC的相互作用，使其对Al的作用更敏感，从而导致其毒性增加。
