Pb2+、Zn2+和Al3+对电压激活钙通道电流的联合作用。
Combined actions of Pb2+, Zn2+, and Al3+ on voltage-activated calcium channel currents.
作者信息
Platt B, Büsselberg D
机构信息
Heinrich-Heine Universität Düsseldorf, Germany.
出版信息
Cell Mol Neurobiol. 1994 Dec;14(6):831-40. doi: 10.1007/BF02088688.
Abstract
- Whole-cell patch clamp experiments were performed on rat dorsal root ganglion (DRG) neurons to investigate the actions of various combinations of Pb2+, Zn2+, and Al3+ on voltage-activated calcium channel currents (VACCCs). 2. Each of these metals has been shown to reduce VACCCs. 3. We investigated the effects of simultaneous application of two cations in the range of their IC50 values. For all possible combinations (Pb2+/Zn2+, Zn2+/Al3+, Al3+/Pb2+), independent of the order of application, we found additive actions on VACCCs. 4. We observed a 75% (+/- 9%) block of the control current when two cations were applied simultaneously. This observation is consistent with both, an action of two metals at the same site as well as independent actions at different locations of the ion channel. 5. The additivity of the effects should be taken into account for questions of public health and the assessment of threshold limits in cases of environmental contamination.
摘要
- 采用全细胞膜片钳实验研究了Pb2+、Zn2+和Al3+的不同组合对大鼠背根神经节（DRG）神经元电压门控性钙通道电流（VACCCs）的作用。2. 已证实这些金属中的每一种都会降低VACCCs。3. 我们研究了在其IC50值范围内同时应用两种阳离子的效果。对于所有可能的组合（Pb2+/Zn2+、Zn2+/Al3+、Al3+/Pb2+），无论应用顺序如何，我们发现对VACCCs有相加作用。4. 当同时应用两种阳离子时，我们观察到对照电流有75%（±9%）的阻断。这一观察结果与两种金属在同一部位起作用以及在离子通道不同位置独立起作用均相符。5. 在公共卫生问题以及环境污染情况下阈值限值的评估中，应考虑到这些效应的相加性。
相似文献
1
Combined actions of Pb2+, Zn2+, and Al3+ on voltage-activated calcium channel currents.Pb2+、Zn2+和Al3+对电压激活钙通道电流的联合作用。
Cell Mol Neurobiol. 1994 Dec;14(6):831-40. doi: 10.1007/BF02088688.
2
Mammalian voltage-activated calcium channel currents are blocked by Pb2+, Zn2+, and Al3+.哺乳动物的电压激活钙通道电流被Pb2+、Zn2+和Al3+阻断。
J Neurophysiol. 1994 Apr;71(4):1491-7. doi: 10.1152/jn.1994.71.4.1491.
3
Mercury (Hg2+) and zinc (Zn2+): two divalent cations with different actions on voltage-activated calcium channel currents.汞（Hg2+）和锌（Zn2+）：两种对电压激活钙通道电流具有不同作用的二价阳离子。
Cell Mol Neurobiol. 1994 Dec;14(6):675-87. doi: 10.1007/BF02088676.
4
Actions of aluminum on voltage-activated calcium channel currents.铝对电压激活钙通道电流的作用。
Cell Mol Neurobiol. 1994 Dec;14(6):819-29. doi: 10.1007/BF02088687.
5
Pb2+ reduces voltage- and N-methyl-D-aspartate (NMDA)-activated calcium channel currents.铅离子（Pb2+）可降低电压门控性和N-甲基-D-天冬氨酸（NMDA）激活的钙通道电流。
Cell Mol Neurobiol. 1994 Dec;14(6):711-22. doi: 10.1007/BF02088679.
6
Zinc (Zn2+) blocks voltage gated calcium channels in cultured rat dorsal root ganglion cells.锌（Zn2+）可阻断培养的大鼠背根神经节细胞中的电压门控钙通道。
Brain Res. 1992 Oct 9;593(1):77-81. doi: 10.1016/0006-8993(92)91266-h.
7
Lead and zinc block a voltage-activated calcium channel of Aplysia neurons.铅和锌会阻断海兔神经元的电压激活钙通道。
J Neurophysiol. 1991 Apr;65(4):786-95. doi: 10.1152/jn.1991.65.4.786.
8
Voltage gated calcium channel currents of rat dorsal root ganglion (DRG) cells are blocked by Al3+.大鼠背根神经节（DRG）细胞的电压门控钙通道电流被Al3 +阻断。
Brain Res. 1993 Sep 17;622(1-2):163-8. doi: 10.1016/0006-8993(93)90815-5.
9
Mercury (Hg2+) decreases voltage-gated calcium channel currents in rat DRG and Aplysia neurons.汞（Hg2+）可降低大鼠背根神经节和海兔神经元中的电压门控钙通道电流。
Brain Res. 1993 Dec 31;632(1-2):121-6. doi: 10.1016/0006-8993(93)91146-j.
10
Voltage-activated calcium channel currents of rat dorsal root ganglion cells are reduced by trimethyl lead.三甲基铅可降低大鼠背根神经节细胞的电压门控性钙通道电流。
Toxicol Lett. 1997 Jul 21;92(2):117-22. doi: 10.1016/s0378-4274(97)00054-4.
引用本文的文献
1
Zinc, Copper, and Calcium: A Triangle in the Synapse for the Pathogenesis of Vascular-Type Senile Dementia.锌、铜、钙：血管性老年痴呆发病机制中的突触三角。
Biomolecules. 2024 Jun 28;14(7):773. doi: 10.3390/biom14070773.
2
Crosstalk of copper and zinc in the pathogenesis of vascular dementia.铜和锌在血管性痴呆发病机制中的相互作用。
J Clin Biochem Nutr. 2022 Jul;71(1):7-15. doi: 10.3164/jcbn.22-40. Epub 2022 Jul 1.
3
Copper as a Collaborative Partner of Zinc-Induced Neurotoxicity in the Pathogenesis of Vascular Dementia.铜作为锌诱导的神经毒性在血管性痴呆发病机制中的协同伙伴。
Int J Mol Sci. 2021 Jul 6;22(14):7242. doi: 10.3390/ijms22147242.
4
MOLECULAR MECHANISMS OF LEAD NEUROTOXICITY.铅神经毒性的分子机制
Adv Neurotoxicol. 2021;5:159-213. doi: 10.1016/bs.ant.2020.11.002. Epub 2021 Feb 17.
5
Metal toxicity at the synapse: presynaptic, postsynaptic, and long-term effects.突触处的金属毒性：突触前、突触后及长期影响。
J Toxicol. 2012;2012:132671. doi: 10.1155/2012/132671. Epub 2012 Jan 12.
6
Link between Aluminum and the Pathogenesis of Alzheimer's Disease: The Integration of the Aluminum and Amyloid Cascade Hypotheses.铝与阿尔茨海默病发病机制之间的联系：铝假说与淀粉样蛋白瀑布假说的整合
Int J Alzheimers Dis. 2011 Mar 8;2011:276393. doi: 10.4061/2011/276393.
本文引用的文献
1
Blockade of mammalian and invertebrate calcium channels by lead.
Neurotoxicology. 1993 Summer-Fall;14(2-3):249-58.
2
Voltage gated calcium channel currents of rat dorsal root ganglion (DRG) cells are blocked by Al3+.大鼠背根神经节（DRG）细胞的电压门控钙通道电流被Al3 +阻断。
Brain Res. 1993 Sep 17;622(1-2):163-8. doi: 10.1016/0006-8993(93)90815-5.
3
Extracellular pH modulates aluminium-blockade of mammalian voltage-activated calcium channel currents.细胞外pH调节哺乳动物电压激活钙通道电流的铝阻断作用。
Neuroreport. 1993 Sep 10;4(11):1251-4. doi: 10.1097/00001756-199309000-00010.
4
Mammalian voltage-activated calcium channel currents are blocked by Pb2+, Zn2+, and Al3+.哺乳动物的电压激活钙通道电流被Pb2+、Zn2+和Al3+阻断。
J Neurophysiol. 1994 Apr;71(4):1491-7. doi: 10.1152/jn.1994.71.4.1491.
5
Behavioral and neurochemical changes in rats simultaneously exposed to manganese and lead.
Arch Toxicol. 1981 Nov;49(1):49-56. doi: 10.1007/BF00352071.
6
Selectivity of the Ca binding site in synaptosome Ca channels. Inhibition of Ca influx by multivalent metal cations.突触体钙通道中钙结合位点的选择性。多价金属阳离子对钙内流的抑制作用。
J Gen Physiol. 1984 Jun;83(6):941-67. doi: 10.1085/jgp.83.6.941.
7
Influx of calcium, strontium, and barium in presynaptic nerve endings.钙、锶和钡流入突触前神经末梢。
J Gen Physiol. 1982 Jun;79(6):1065-87. doi: 10.1085/jgp.79.6.1065.
8
Activation of calmodulin by various metal cations as a function of ionic radius.不同金属阳离子对钙调蛋白的激活作用与离子半径的关系。
Mol Pharmacol. 1984 Jul;26(1):75-82.
9
Biological membranes: the physical basis of ion and nonelectrolyte selectivity.生物膜：离子与非电解质选择性的物理基础。
Annu Rev Physiol. 1969;31:581-646. doi: 10.1146/annurev.ph.31.030169.003053.
10
Zinc deficiency in the postnatal rat: implications for lead toxicity.出生后大鼠的锌缺乏：对铅毒性的影响。
Neurotoxicology. 1986 Spring;7(1):237-46.
Zotero 插件